Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot analysis of GPx-2 expression in HepG2 (A), mouse stomach (B), rat small intestine (C) whole cell lysates.)

Rabbit GPx-2 Polyclonal Antibody | anti-GPx-2 antibody

Anti-GPx-2 Antibody

Gene Names
GPX2; GPRP; GPx-2; GI-GPx; GPRP-2; GPx-GI; GSHPx-2; GSHPX-GI
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Purified by Immunogen Affinity Chromatography
Synonyms
GPx-2; Polyclonal Antibody; Anti-GPx-2 Antibody; Glutathione peroxidase 2; GSHPx-2; Gastrointestinal glutathione peroxidase; Glutathione peroxidase-gastrointestinal; GPx-GI; GSHPx-GI; Glutathione peroxidase-related protein 2; GPRP-2; anti-GPx-2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of GPx-2 protein.
Purity/Purification
Purified by Immunogen Affinity Chromatography
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Applicable Applications for anti-GPx-2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
Recombinant fusion protein of human GPx-2. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of GPx-2 expression in HepG2 (A), mouse stomach (B), rat small intestine (C) whole cell lysates.)

Western Blot (WB) (Western blot analysis of GPx-2 expression in HepG2 (A), mouse stomach (B), rat small intestine (C) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of GPx-2 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of GPx-2 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-GPx-2 antibody
Rabbit polyclonal antibody to GPx-2

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,954 Da
NCBI Official Full Name
glutathione peroxidase 2
NCBI Official Synonym Full Names
glutathione peroxidase 2
NCBI Official Symbol
GPX2
NCBI Official Synonym Symbols
GPRP; GPx-2; GI-GPx; GPRP-2; GPx-GI; GSHPx-2; GSHPX-GI
NCBI Protein Information
glutathione peroxidase 2; gastrointestinal glutathione peroxidase; glutathione peroxidase 2 (gastrointestinal); glutathione peroxidase-related protein 2
UniProt Protein Name
Glutathione peroxidase 2
UniProt Gene Name
GPX2
UniProt Synonym Gene Names
GPx-2; GSHPx-2; GPx-GI; GSHPx-GI; GPRP-2
UniProt Entry Name
GPX2_HUMAN

NCBI Description

This gene is a member of the glutathione peroxidase family and encodes a selenium-dependent glutathione peroxidase that is one of two isoenzymes responsible for the majority of the glutathione-dependent hydrogen peroxide-reducing activity in the epithelium of the gastrointestinal tract. The protein encoded by this locus contains a selenocysteine (Sec) residue encoded by the UGA codon, which normally signals translation termination. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]

Uniprot Description

GPX2: Could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors. Belongs to the glutathione peroxidase family.

Protein type: EC 1.11.1.9; Oxidoreductase; Other Amino Acids Metabolism - glutathione; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 14q24.1

Cellular Component: cytoplasm; cytosol

Molecular Function: electron carrier activity; glutathione peroxidase activity

Biological Process: response to reactive oxygen species; response to symbiotic bacterium; negative regulation of inflammatory response to antigenic stimulus; thermoregulation; interaction with symbiont

Research Articles on GPx-2

Similar Products

Product Notes

The GPx-2 gpx2 (Catalog #AAA8306817) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GPx-2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GPx-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the GPx-2 gpx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPx-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.