Rabbit IL-16 Polyclonal Antibody | anti-IL16 antibody

IL-16 Antibody

Cat. Num. AAA151374

• Gene Names: IL16; LCF; NIL16; PRIL16; prIL-16
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: IL-16 Antibody is affinity chromatography purified via peptide column.
• Synonyms: IL-16; Polyclonal Antibody; IL-16 Antibody; LCF; NIL16; PRIL16; prIL-16; Pro-interleukin-16; Lymphocyte chemoattractant factor; interleukin 16 (lymphocyte chemoattractant factor); anti-IL16 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody will only detect the NIL-16 isoform.
• Purity/Purification: IL-16 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1332

• Applicable Applications for anti-IL16 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IL-16 antibody can be used for detection of IL-16 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: IL-16 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human IL-16.
• Buffer: IL-16 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: IL-16 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of IL-16 in rat brain tissue lysate with IL-16 antibody at (A) 2.5 and (B) 5 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of IL-16 in mouse brain tissue with IL-16 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of IL-16 in mouse brain tissue with IL-16 antibody at 20 μg/mL.)

Related Product Information for anti-IL16 antibody

IL-16 Antibody: IL-16 was initially identified as a chemotactic cytokine, but is now known to possess a wide range of activities. Later studies have more fully characterized IL-16 as an immunomodulatory cytokine that contributes to the regulatory process of CD4+ T cell recruitment and activation at sites of inflammation in association with asthma and several autoimmune diseases. The precursor of IL-16 (pro-IL-16) is thought to be cleaved towards the C-terminal region by Caspase-3, releasing a 20 kDa active form that binds to and signals through CD4. Besides acting as a chemotactic cytokine, IL-16 is thought to also be involved in the regulation of T cell proliferation and multiple infectious, immune-mediated, and autoimmune inflammatory disorders including irritable bowel syndrome, systemic lupus erythematosus, and neurodegenerative disorders. At least two isoforms of IL-16 are known to exist; the longer isoform (also known as NIL-16) is detected only in neurons of the cerebellum and hippocampus.

NCBI and Uniprot Product Information

• NCBI GI #: 36953836
• NCBI GeneID: 3603
• UniProt Accession #: Q14005; Q16435; Q6VVE6; Q6ZMQ7; Q9UP18; A6NM20; A8MU65; B5TY35; B9EGR6; H3BVH5
• Molecular Weight: 59,345 Da
• NCBI Official Full Name: neural interleukin 16 protein
• NCBI Official Synonym Full Names: interleukin 16
• NCBI Official Symbol: IL16
• NCBI Official Synonym Symbols: LCF; NIL16; PRIL16; prIL-16
• NCBI Protein Information: pro-interleukin-16; prointerleukin 16; neuronal interleukin 16; lymphocyte chemoattractant factor
• UniProt Protein Name: Pro-interleukin-16
• Protein Family: Pro-interleukin
• UniProt Gene Name: IL16
• UniProt Synonym Gene Names: IL-16; LCF
• UniProt Entry Name: IL16_HUMAN

NCBI Description

The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Uniprot Description

IL16: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Cytokine; Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: extracellular space; cytoplasm; plasma membrane; nucleus

Molecular Function: cytokine activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; viral reproduction; leukocyte chemotaxis; immune response; induction of positive chemotaxis