Western Blot (WB)
(Western blot analysis of GNPDA1 in mouse kidney lysate with GNPDA1 antibody at (A) 1 and (B) 2 μg/mL.)
Rabbit GNPDA1 Polyclonal Antibody | anti-GNPDA1 antibody
GNPDA1 Antibody
Gene Names
GNPDA1; GPI; HLN; GNP1; GNPI; GNPDA
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
GNPDA1 Antibody is affinity chromatography purified via peptide column.
Synonyms
GNPDA1; Polyclonal Antibody; GNPDA1 Antibody; GPI; HLN; GNP1; GNPI; GNPDA; KIAA0060; Glucosamine-6-phosphate isomerase 1; Glucosamine-6-phosphate deaminase 1; GNPDA 1; glucosamine-6-phosphate deaminase 1; anti-GNPDA1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
GNPDA1 is predicted to not cross-react with GNPDA2.
Purity/Purification
GNPDA1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
289
Applicable Applications for anti-GNPDA1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
GNPDA1 antibody can be used for detection of GNPDA1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
GNPDA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human GNPDA1.
Buffer
GNPDA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
GNPDA1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of GNPDA1 in mouse kidney lysate with GNPDA1 antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of GNPDA1 in mouse kidney lysate with GNPDA1 antibody at (A) 1 and (B) 2 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of GNPDA1 in 293 cells with GNPDA1 antibody at 5 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of GNPDA1 in 293 cells with GNPDA1 antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of GNPDA1 in 293 cells with GNPDA1 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of GNPDA1 in 293 cells with GNPDA1 antibody at 20 μg/mL.)
Related Product Information for anti-GNPDA1 antibody
GNPDA1 Antibody: Glucosamine-6-phosphate deaminase (GNPDA) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. GNPDA1 is one of two mammalian glucosamine-6-phosphate deaminases that are thought to have arisen though gene duplication, with the GNPDA2 protein closer in structure and activity to the E. coli enzyme. GNPDA1 possesses greater affinity for ammonium than either GNPDA2 or the E. coli enzyme suggesting that the forward reaction of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium is catalyzed at a slower rate than GNPDA2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
24,047 Da
NCBI Official Full Name
glucosamine-6-phosphate deaminase
NCBI Official Synonym Full Names
glucosamine-6-phosphate deaminase 1
NCBI Official Symbol
GNPDA1
NCBI Official Synonym Symbols
GPI; HLN; GNP1; GNPI; GNPDA
NCBI Protein Information
glucosamine-6-phosphate isomerase 1; GNPDA 1; oscillin; glcN6P deaminase 1
UniProt Protein Name
Glucosamine-6-phosphate isomerase 1
Protein Family
UniProt Gene Name
GNPDA1
UniProt Synonym Gene Names
GNPI; HLN; KIAA0060; GNPDA 1; GlcN6P deaminase 1
UniProt Entry Name
GNPI1_HUMAN
NCBI Description
Glucosamine-6-phosphate deaminase (EC 3.5.99.6) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium (Arreola et al., 2003 [PubMed 12965206]).[supplied by OMIM, Jan 2010]
Uniprot Description
GNPDA1: Seems to trigger calcium oscillations in mammalian eggs. These oscillations serve as the essential trigger for egg activation and early development of the embryo. Belongs to the glucosamine/galactosamine-6-phosphate isomerase family.
Protein type: EC 3.5.99.6; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Isomerase; Hydrolase
Chromosomal Location of Human Ortholog: 5q21
Cellular Component: cytoplasm
Molecular Function: glucosamine-6-phosphate deaminase activity; hydrolase activity
Biological Process: generation of precursor metabolites and energy; single fertilization; glucosamine catabolic process; carbohydrate metabolic process; N-acetylglucosamine metabolic process
Research Articles on GNPDA1
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Product Notes
The GNPDA1 gnpda1 (Catalog #AAA150944) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GNPDA1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GNPDA1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). GNPDA1 antibody can be used for detection of GNPDA1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the GNPDA1 gnpda1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GNPDA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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