Western Blot (WB)
(GNPDA1 western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the GNPDA1 antibody detected the GNPDA1 protein (arrow).)
Mouse anti-Mouse GNPDA1 Monoclonal Antibody | anti-GNPDA1 antibody
GNPDA1, NT (Glucosamine-6-phosphate Deaminase 1, Glucosamine-6-phosphate Isomerase 1, GlcN6P Deaminase 1, Oscillin, GNPI, HLN, KIAA0060) (AP)
Gene Names
GNPDA1; GPI; HLN; GNP1; GNPI; GNPDA
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
GNPDA1; Monoclonal Antibody; NT (Glucosamine-6-phosphate Deaminase 1; Glucosamine-6-phosphate Isomerase 1; GlcN6P Deaminase 1; Oscillin; GNPI; HLN; KIAA0060) (AP); anti-GNPDA1 antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
237CT2.5.2
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-GNPDA1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: 1:1000
WB: 1:100-1000
Applications are based on unconjugated antibody.
WB: 1:100-1000
Applications are based on unconjugated antibody.
Immunogen
GNPDA1 Mab is generated from mouse immunized with GNPDA1 recombinant protein.
Conjugate
AP
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C with the addition of glycerol. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive.
Western Blot (WB)
(GNPDA1 western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the GNPDA1 antibody detected the GNPDA1 protein (arrow).)
Western Blot (WB)
(GNPDA1 western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the GNPDA1 antibody detected the GNPDA1 protein (arrow).)
Related Product Information for anti-GNPDA1 antibody
Glucosamine-6-phosphate deaminase (EC 3.5.99.6) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium (Arreola et al., 2003 [PubMed 12965206]).
Product Categories/Family for anti-GNPDA1 antibody
References
Lamesch, P., et al. Genomics 89(3):307-315(2007) Arreola, R., et al. FEBS Lett. 551 (1-3), 63-70 (2003) Zhang, J., et al. J. Cell. Biochem. 88(5):932-940(2003) Nakamura, Y., et al. Genomics 68(2):179-186(2000) Shevchenko, V., et al. Gene 216(1):31-38(1998)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,047 Da
NCBI Official Full Name
glucosamine-6-phosphate isomerase 1
NCBI Official Synonym Full Names
glucosamine-6-phosphate deaminase 1
NCBI Official Symbol
GNPDA1
NCBI Official Synonym Symbols
GPI; HLN; GNP1; GNPI; GNPDA
NCBI Protein Information
glucosamine-6-phosphate isomerase 1; GNPDA 1; glcN6P deaminase 1; oscillin
UniProt Protein Name
Glucosamine-6-phosphate isomerase 1
Protein Family
UniProt Gene Name
GNPDA1
UniProt Synonym Gene Names
GNPI; HLN; KIAA0060; GNPDA 1; GlcN6P deaminase 1
UniProt Entry Name
GNPI1_HUMAN
NCBI Description
Glucosamine-6-phosphate deaminase (EC 3.5.99.6) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium (Arreola et al., 2003 [PubMed 12965206]).[supplied by OMIM, Jan 2010]
Uniprot Description
GNPDA1: Seems to trigger calcium oscillations in mammalian eggs. These oscillations serve as the essential trigger for egg activation and early development of the embryo. Belongs to the glucosamine/galactosamine-6-phosphate isomerase family.
Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; Isomerase; EC 3.5.99.6; Hydrolase
Chromosomal Location of Human Ortholog: 5q21
Cellular Component: cytoplasm
Molecular Function: glucosamine-6-phosphate deaminase activity; hydrolase activity
Biological Process: generation of precursor metabolites and energy; single fertilization; N-acetylglucosamine metabolic process; carbohydrate metabolic process; glucosamine catabolic process
Research Articles on GNPDA1
Similar Products
Product Notes
The GNPDA1 gnpda1 (Catalog #AAA6120834) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GNPDA1, NT (Glucosamine-6-phosphate Deaminase 1, Glucosamine-6-phosphate Isomerase 1, GlcN6P Deaminase 1, Oscillin, GNPI, HLN, KIAA0060) (AP) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GNPDA1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 1:1000 WB: 1:100-1000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GNPDA1 gnpda1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GNPDA1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
