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Western Blot (WB) (Western blot analysis of GNPDA2 in human kidney lysate with GNPDA2 antibody at 1 μg/mL.)

Rabbit anti-Human, Mouse GNPDA2 Polyclonal Antibody | anti-GNPDA2 antibody

GNPDA2 Antibody

Gene Names
GNPDA2; GNP2; SB52
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
GNPDA2 Antibody is affinity chromatography purified via peptide column.
Synonyms
GNPDA2; Polyclonal Antibody; GNPDA2 Antibody; GNP2; SB52; Glucosamine-6-phosphate isomerase 2; Glucosamine-6-phosphate deaminase 2; GNPDA 2; glucosamine-6-phosphate deaminase 2; anti-GNPDA2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
GNPDA2 antibody is predicted to not cross-react with GNPDA1.
Purity/Purification
GNPDA2 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
276
Applicable Applications for anti-GNPDA2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
GNPDA2 antibody can be used for detection of GNPDA2 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 4 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
GNPDA2 antibody was raised against an 8 amino acid synthetic peptide near the carboxy terminus of human GNPDA2.
Buffer
GNPDA2 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
GNPDA2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of GNPDA2 in human kidney lysate with GNPDA2 antibody at 1 μg/mL.)

Western Blot (WB) (Western blot analysis of GNPDA2 in human kidney lysate with GNPDA2 antibody at 1 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of GNPDA2 in 293 cells with GNPDA2 antibody at 4 μg/mL.)

Immunocytochemistry (IHC) (Immunocytochemistry of GNPDA2 in 293 cells with GNPDA2 antibody at 4 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of GNPDA2 in mouse kidney tissue with GNPDA2 antibody at 20 μg/mL.)

Immunofluorescence (IF) (Immunofluorescence of GNPDA2 in mouse kidney tissue with GNPDA2 antibody at 20 μg/mL.)
Related Product Information for anti-GNPDA2 antibody
GNPDA2 Antibody: Glucosamine-6-phosphate deaminase (GNPDA) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. GNPDA2 is the second mammalian glucosamine-6-phosphate deaminase discovered, and is closer in structure and activity to the E. coli enzyme than GNPDA1. GNPDA1 possesses greater affinity for ammonium than either GNPDA2 or the E. coli enzyme suggesting that the forward reaction of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium is catalyzed at a slower rate than GNPDA2.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,091 Da
NCBI Official Full Name
glucosamine-6-phosphate isomerase 2 isoform 1
NCBI Official Synonym Full Names
glucosamine-6-phosphate deaminase 2
NCBI Official Symbol
GNPDA2
NCBI Official Synonym Symbols
GNP2; SB52
NCBI Protein Information
glucosamine-6-phosphate isomerase 2; glcN6P deaminase 2; glucosamine-6-phosphate isomerase SB52
UniProt Protein Name
Glucosamine-6-phosphate isomerase 2
UniProt Gene Name
GNPDA2
UniProt Synonym Gene Names
GNP2; GNPDA 2; GlcN6P deaminase 2
UniProt Entry Name
GNPI2_HUMAN

NCBI Description

The protein encoded by this gene is an allosteric enzyme that catalyzes the reversible reaction converting D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. Variations of this gene have been reported to be associated with influencing body mass index and susceptibility to obesity. A pseudogene of this gene is located on chromosome 9. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]

Uniprot Description

GNPDA2: is an allosteric enzyme that catalyzes the reversible reaction converting D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. Variations of this gene have been reported to be associated with influencing body mass index and susceptibility to obesity. A pseudogene of this gene is located on chromosome 9. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]

Protein type: Isomerase; EC 3.5.99.6; Hydrolase; Carbohydrate Metabolism - amino sugar and nucleotide sugar

Chromosomal Location of Human Ortholog: 4p12

Cellular Component: cytoplasm; nucleus

Molecular Function: glucosamine-6-phosphate deaminase activity; hydrolase activity

Biological Process: carbohydrate metabolic process; N-acetylglucosamine metabolic process

Research Articles on GNPDA2

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Product Notes

The GNPDA2 gnpda2 (Catalog #AAA150148) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GNPDA2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GNPDA2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). GNPDA2 antibody can be used for detection of GNPDA2 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 4 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the GNPDA2 gnpda2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GNPDA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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