Mouse anti-Human ADRA1A Monoclonal Antibody | anti-ADRA1A antibody

ADRA1A (Alpha-1A Adrenergic Receptor, Alpha-1A Adrenoreceptor, Alpha-1A Adrenoceptor, Alpha-1C Adrenergic Receptor, Alpha-adrenergic Receptor 1c, ADRA1C) (Biotin)

Cat. Num. AAA6140507

• Gene Names: ADRA1A; ADRA1C; ADRA1L1; ALPHA1AAR
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ADRA1A; Monoclonal Antibody; ADRA1A (Alpha-1A Adrenergic Receptor; Alpha-1A Adrenoreceptor; Alpha-1A Adrenoceptor; Alpha-1C Adrenergic Receptor; Alpha-adrenergic Receptor 1c; ADRA1C) (Biotin); anti-ADRA1A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4C7
• Specificity: Recognizes human ADRA1A.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ADRA1A antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa1-27 from human ADRA1A (NP_150646) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MVFLSGNASDSSNCTQPPAPVNISKAI
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (28.71kD).)

Western Blot (WB)

(ADRA1A monoclonal antibody Western Blot analysis of ADRA1A expression in HL-60.)

Testing Data

(Detection limit for recombinant GST tagged ADRA1A is ~0.3ng/ml as a capture antibody.)

Related Product Information for anti-ADRA1A antibody

This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes.

Product Categories/Family for anti-ADRA1A antibody

Antibodies; Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 111118986
• NCBI GeneID: 148
• NCBI Accession #: NP_150646
• NCBI GenBank Nucleotide #: NM_033303
• Molecular Weight: 40,638 Da
• NCBI Official Full Name: alpha-1A adrenergic receptor isoform 2
• NCBI Official Synonym Full Names: adrenoceptor alpha 1A
• NCBI Official Symbol: ADRA1A
• NCBI Official Synonym Symbols: ADRA1C; ADRA1L1; ALPHA1AAR
• NCBI Protein Information: alpha-1A adrenergic receptor
• Protein Family: Alpha-1A adrenergic receptor

NCBI Description

Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq, Jul 2008]