Rabbit anti-Human KPNA5 Polyclonal Antibody | anti-KPNA5 antibody

KPNA5 Antibody

Cat. Num. AAA150876

• Gene Names: KPNA5; SRP6; IPOA6
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: KPNA5 Antibody is affinity chromatography purified via peptide column.
• Synonyms: KPNA5; Polyclonal Antibody; KPNA5 Antibody; SRP6; IPOA6; Importin subunit alpha-6; Karyopherin subunit alpha-5; karyopherin alpha 5 (importin alpha 6); anti-KPNA5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: KPNA5 antibody is human specific. KPNA5 antibody is predicted to not cross-react with other Importin alpha family members.
• Purity/Purification: KPNA5 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 536

• Applicable Applications for anti-KPNA5 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: KPNA5 antibody can be used for detection of KPNA5 by immunohistochemistry at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: KPNA5 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human KPNA5.
• Buffer: KPNA5 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: KPNA5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Immunohistochemistry (IHC)

(Immunohistochemistry of KPNA5 in human testis tissue with KPNA5 antibody at 5 μg/mL.)

Related Product Information for anti-KPNA5 antibody

KPNA5 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA5 belongs to a subfamily within the KPNA family that also includes KPNA4 and 6 and is thought to be involved in NLS-dependent protein import into the nucleus.

NCBI and Uniprot Product Information

• NCBI GI #: 55664992
• NCBI GeneID: 3841
• UniProt Accession #: O60684; Q5VVU3; B2RDC7; D3DPP5; O15131
• Molecular Weight: 60,030 Da
• NCBI Official Synonym Full Names: karyopherin alpha 5 (importin alpha 6)
• NCBI Official Symbol: KPNA5
• NCBI Official Synonym Symbols: SRP6; IPOA6
• NCBI Protein Information: importin subunit alpha-6
• UniProt Protein Name: Importin subunit alpha-7
• UniProt Gene Name: KPNA6
• UniProt Synonym Gene Names: IPOA7
• UniProt Entry Name: IMA7_HUMAN

NCBI Description

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus. [provided by RefSeq, Jul 2008]

Uniprot Description

KPNA6: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Belongs to the importin alpha family.

Protein type: Karyopherin; Nuclear import

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: membrane; cytoplasm; nucleus

Molecular Function: protein transporter activity

Biological Process: maternal process involved in pregnancy; positive regulation of transcription from RNA polymerase II promoter; NLS-bearing substrate import into nucleus