Rabbit KPNA6 Polyclonal Antibody | anti-KPNA6 antibody

KPNA6 Antibody

Cat. Num. AAA150717

• Gene Names: KPNA6; IPOA7; KPNA7
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: KPNA6 Antibody is affinity chromatography purified via peptide column.
• Synonyms: KPNA6; Polyclonal Antibody; KPNA6 Antibody; IPOA7; KPNA7; Importin subunit alpha-7; Karyopherin subunit alpha-6; karyopherin alpha 6 (importin alpha 7); anti-KPNA6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: KPNA6 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 536

• Applicable Applications for anti-KPNA6 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: KPNA6 antibody can be used for detection of KPNA6 by Western blot at 1 ug/mL. Antibody can also be used for immunofluorescence starting at 20 ug/mL.For immunofluorescence start at 20 ug/mL.; ; Optiaml dilutions for each applciation to be determined by the researcher.
• Homology: Predicted species reactivity bases on immunogen sequence : Bovine (92%)
• Conjugate: Unconjugated
• Immunogen: KPNA6 antibody was raised against a 13 amino acid synthetic peptide near the amino terminus of human KPNA6. The immunogen is located within amino acids 70-120 of KPNA6.
• Buffer: KPNA6 Antibody is supplied in PBS containing 0.02% sodium azide.
• Positive Control: MBS151597- HEK293 Cell Lysate; MBS154185-HEK293 Cell Slide.
• Preparation and Storage: KPNA6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of KPNA6 in 293 cell lysate with KPNA6 antibody at 1 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of KPNA6 in 293 cells with KPNA6 antibody at 20 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of KPNA6 in 293 cells with KPNA6 antibody at 20 μg/mL.)

Related Product Information for anti-KPNA6 antibody

KPNA6 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA6 belongs to a subfamily within the KPNA family that also includes IPOA5&6. Down-regulation of KPNA6 by RNAi expression in HeLa cells strongly inhibited cell proliferation, possibly due to blocking the nuclear import of specific factors essential for cell growth and proliferation. KPNA6 also interacts with the Rev protein of HIV-1 and promote its nuclear import.

NCBI and Uniprot Product Information

• NCBI GI #: 55664992
• NCBI GeneID: 23633
• UniProt Accession #: O60684; Q5VVU3; B2RDC7; D3DPP5
• NCBI Official Synonym Full Names: karyopherin alpha 6 (importin alpha 7)
• NCBI Official Symbol: KPNA6
• NCBI Official Synonym Symbols: IPOA7; KPNA7
• NCBI Protein Information: importin subunit alpha-7
• UniProt Protein Name: Importin subunit alpha-7
• UniProt Gene Name: KPNA6
• UniProt Synonym Gene Names: IPOA7
• UniProt Entry Name: IMA7_HUMAN

NCBI Description

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. The protein encoded by this gene is a member of the importin alpha family. [provided by RefSeq, Jul 2008]

Uniprot Description

KPNA6: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Belongs to the importin alpha family.

Protein type: Karyopherin; Nuclear import

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: membrane; cytoplasm; nucleus

Molecular Function: protein transporter activity

Biological Process: maternal process involved in pregnancy; positive regulation of transcription from RNA polymerase II promoter; NLS-bearing substrate import into nucleus