KPNA5 blocking peptide

KPNA5 Peptide

Cat. Num. AAA152567

• Gene Names: KPNA5; SRP6; IPOA6
• Applications: Blocking (BL)
• Synonyms: KPNA5; KPNA5 Peptide; SRP6; IPOA6; Importin subunit alpha-6; Karyopherin subunit alpha-5; karyopherin alpha 5 (importin alpha 6); KPNA5 blocking peptide

• Form/Format: Liquid
• Concentration: 200 ug/mL (varies by lot)
• Sequence Length: 536

• Applicable Applications for KPNA5 blocking peptide: Blocking (BL)
• Application Notes: KPNA5 peptide is used for blocking the activity of KPNA5 antibody.
• Species: Human
• Buffer: PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide
• Location: 18 amino acids near the amino terminus of human KPNA5.
• Preparation and Storage: Store KPNA5 peptide at -20 degree C, stable for one year.

Western Blot (WB)

(Western blot analysis of KPNA6 in EL4 cell lysate with KPNA5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 55664992
• NCBI GeneID: 3841
• UniProt Accession #: O60684; Q5VVU3; B2RDC7; D3DPP5; O15131
• Molecular Weight: 60,030 Da
• NCBI Official Synonym Full Names: karyopherin alpha 5 (importin alpha 6)
• NCBI Official Symbol: KPNA5
• NCBI Official Synonym Symbols: SRP6; IPOA6
• NCBI Protein Information: importin subunit alpha-6
• UniProt Protein Name: Importin subunit alpha-7
• UniProt Gene Name: KPNA6
• UniProt Synonym Gene Names: IPOA7
• UniProt Entry Name: IMA7_HUMAN

NCBI Description

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus. [provided by RefSeq, Jul 2008]

Uniprot Description

KPNA6: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Belongs to the importin alpha family.

Protein type: Nuclear import; Karyopherin

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: membrane; cytoplasm; nucleus

Molecular Function: protein transporter activity

Biological Process: maternal process involved in pregnancy; positive regulation of transcription from RNA polymerase II promoter; NLS-bearing substrate import into nucleus