Rabbit anti-Human, Mouse IKAP Polyclonal Antibody | anti-IKBKAP antibody

IKAP Antibody

Cat. Num. AAA150596

• Gene Names: IKBKAP; FD; DYS; ELP1; IKAP; IKI3; TOT1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: IKAP Antibody is affinity chromatography purified via peptide column.
• Synonyms: IKAP; Polyclonal Antibody; IKAP Antibody; FD; DYS; ELP1; IKI3; TOT1; Elongator complex protein 1; IkappaB kinase complex-associated protein; inhibitor of kappa light polypeptide gene enhancer in B-cells; kinase complex-associated protein; anti-IKBKAP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: IKAP Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 1332

• Applicable Applications for anti-IKBKAP antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IKAP antibody can be used for detection of IKAP by Western blot at 0.5 to 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 1 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: IKAP antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human IKAP.
• Buffer: IKAP Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: IKAP antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of IKAP in A-20 cell lysate with IKAP antibody at in (A) 0.5, and (B) 1 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of IKAP in A-20 cells with IKAP antibody at 1 μg/mL. )

Immunofluorescence (IF)

(Immunofluorescence of IKAP in A20 cells with IKAP antibody at 20 μg/mL.)

Related Product Information for anti-IKBKAP antibody

IKAP Antibody: IKAP was initially identified as a scaffold protein of the IκB kinase complex that could bind to IKKα, IKKβ, NF-κB, and the NF-κB-inducing kinase (NIK), although later evidence has cast doubt on this. More recent reports show that mutations in IKAP such as a frameshift leading to a truncated protein or a missense mutation that leads to defective phosphorylation are responsible for the autosomal recessive genetic disease familial dysautonomia (FD). Reports indicating that it forms part of the RNA polymerase II transcription elongation complex suggest that this disease may be due to compromised transcription elongation. More recently, it was shown that IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD. At least two isoforms of IKAP are known two exist.

NCBI and Uniprot Product Information

• NCBI GI #: 3757822
• NCBI GeneID: 8518
• UniProt Accession #: O95163; Q5JSV2; Q9H327; Q9UG87
• Molecular Weight: 150,254 Da
• NCBI Official Full Name: IkappaB kinase complex associated protein
• NCBI Official Synonym Full Names: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein
• NCBI Official Symbol: IKBKAP
• NCBI Official Synonym Symbols: FD; DYS; ELP1; IKAP; IKI3; TOT1
• NCBI Protein Information: elongator complex protein 1; p150; IKK complex-associated protein; ikappaB kinase complex-associated protein; elongator acetyltransferase complex subunit 1
• UniProt Protein Name: Elongator complex protein 1
• UniProt Gene Name: IKBKAP
• UniProt Synonym Gene Names: ELP1; IKAP; ELP1; IKK complex-associated protein
• UniProt Entry Name: ELP1_HUMAN

NCBI Description

The protein encoded by this gene is a scaffold protein and a regulator for 3 different kinases involved in proinflammatory signaling. This encoded protein can bind NF-kappa-B-inducing kinase (NIK) and IKKs through separate domains and assemble them into an active kinase complex. Mutations in this gene have been associated with familial dysautonomia. [provided by RefSeq, Jul 2008]

Uniprot Description

IKBKAP: May act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK). Interacts preferentially with MAP3K14/NIK followed by IKK-alpha and IKK-beta. Component of the RNA polymerase II elongator complex (Elongator), which consists of IKBKAP/ELP1, STIP1/ELP2, ELP3, ELP4, and two yet unidentified proteins, p30 and p38. Elongator associates with the C-terminal domain (CTD) of Pol II largest subunit. Interacts with ELP3. Belongs to the ELP1/IKA1 family.

Protein type: Protein kinase, regulatory subunit; Transcription regulation

Chromosomal Location of Human Ortholog: 9q31

Cellular Component: transcription elongation factor complex; Elongator holoenzyme complex; cytoplasm; nucleolus; histone acetyltransferase complex

Molecular Function: protein binding; signal transducer activity; phosphorylase kinase regulator activity

Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; RNA elongation from RNA polymerase II promoter; immune response; protein complex assembly; signal transduction; regulation of protein kinase activity; protein amino acid phosphorylation; positive regulation of cell migration

Disease: Neuropathy, Hereditary Sensory And Autonomic, Type Iii