Rabbit anti-Human IKAP Polyclonal Antibody | anti-IKBKAP antibody

IKAP, CT (IkB Associated Protein)

Cat. Num. AAA648149

• Gene Names: IKBKAP; FD; DYS; ELP1; IKAP; IKI3; TOT1
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: IKAP; Polyclonal Antibody; CT (IkB Associated Protein); Anti -IKAP; anti-IKBKAP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IKAP.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid.

• Applicable Applications for anti-IKBKAP antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: C-terminus of IKAP
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot of IKAP (1:500) in HEK293 cells stimulated with interleukin-1.)

Product Categories/Family for anti-IKBKAP antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 3757822
• NCBI GeneID: 8518
• UniProt Accession #: O95163; Q5JSV2; Q9H327; Q9UG87
• Molecular Weight: 150,254 Da
• NCBI Official Full Name: IkappaB kinase complex associated protein
• NCBI Official Synonym Full Names: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein
• NCBI Official Symbol: IKBKAP
• NCBI Official Synonym Symbols: FD; DYS; ELP1; IKAP; IKI3; TOT1
• NCBI Protein Information: elongator complex protein 1; p150; IKK complex-associated protein; ikappaB kinase complex-associated protein; elongator acetyltransferase complex subunit 1
• UniProt Protein Name: Elongator complex protein 1
• UniProt Gene Name: IKBKAP
• UniProt Synonym Gene Names: ELP1; IKAP; ELP1
• UniProt Entry Name: ELP1_HUMAN

NCBI Description

The protein encoded by this gene is a scaffold protein and a regulator for 3 different kinases involved in proinflammatory signaling. This encoded protein can bind NF-kappa-B-inducing kinase (NIK) and IKKs through separate domains and assemble them into an active kinase complex. Mutations in this gene have been associated with familial dysautonomia. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: May act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK). Ref.7 Ref.8Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. Involved in cell migration. Ref.7 Ref.8

Subunit structure: Interacts preferentially with MAP3K14/NIK followed by IKK-alpha and IKK-beta. Component of the RNA polymerase II elongator complex (Elongator), which consists of IKBKAP/ELP1, STIP1/ELP2, ELP3, ELP4, ELP5 and ELP6. Elongator associates with the C-terminal domain (CTD) of Pol II largest subunit. Interacts with ELP3. Ref.7 Ref.8 Ref.12

Subcellular location: Cytoplasm. Nucleus Ref.7 Ref.8 Ref.12.

Involvement in disease: Hereditary sensory and autonomic neuropathy 3 (HSAN3) [MIM:223900]: A form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN3 patients manifest a variety of symptoms such as alacrima, decreased taste, decreased sensitivity to pain and temperature, vasomotor instability, hypoactive or absent deep tendon reflexes, vomiting crises, and gastrointestinal dysfunction.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.2 Ref.13

Sequence similarities: Belongs to the ELP1/IKA1 family.

Sequence caution: The sequence CAB43219.1 differs from that shown. Reason: Frameshift at position 1286.