Mouse IKAP Monoclonal Antibody | anti-IKAP antibody

IKAP (Central region) Antibody

Cat. Num. AAA474394

• Gene Names: IKBKAP; FD; DYS; ELP1; IKAP; IKI3; TOT1
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA
• Purity: Protein A chromatography
• Synonyms: IKAP; Monoclonal Antibody; IKAP (Central region) Antibody; IKAP (Central region); anti-IKAP antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M368
• Specificity: The antibody detects a 150kDa* band corresponding to the molecular weight of IKAP on SDS-PAGE immunoblots of Jurkat cells.
• Purity/Purification: Protein A chromatography
• Form/Format: Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-IKAP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: 1:2000; WB: 1:1000
• Immunogen: Clone M368 was generated from a recombinant protein that included amino acid residues within the central region of human IKAP. This sequence has high homology with similar regions in rat and mouse IKAP.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot analysis of IKAP expression in human Jurkat cell lysate. The blot was probed with mouse monoclonal IKAP (Central region) at 1:500 (lane 1) or 1:2000 (lane 2).)

Related Product Information for anti-IKAP antibody

The NF-k B transcription factor coordinates a response to pathogens and pro-inflammatory cytokines, and is vital in the development of acute and chronic inflammatory diseases. NF-k B is activated by cytokine-activated IKB kinases (IKKs) found in large complexes. The IKK complexes consist of NIK, IKK-a, IKK-b, IkB-a, NF-k B/RelA and IKK-complex-associated protein (IKAP). First identified as a scaffolding protein of the IKK complex, IKAP, also known as elongation protein 1 (ELP1), has been linked to adhesion and migration in several cell types, including rat primary neurons. Depletion of IKAP by loss-of-function mutations in the IKBKAP gene has been shown to cause defective neuronal development and maintenance, leading to familial dysautonomia (FD). FD is a hereditary neuronal disease characterized by poor development and progressive degeneration of the sensory and autonomic nervous system. In addition, IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD.

References

Johansen, L. D. et al. (2008) Journal of Cell Science 121:854.
Naumanen, T. et al. (2008) Cel. Adh. Migr. 2(4):236.
Cohen, L. et al. (1998) Nature 395:292.

NCBI and Uniprot Product Information

• NCBI GI #: 3757822
• NCBI GeneID: 8518
• UniProt Accession #: O95163; Q5JSV2; Q9H327; Q9UG87
• Molecular Weight: 150,254 Da
• NCBI Official Full Name: IkappaB kinase complex associated protein
• NCBI Official Synonym Full Names: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein
• NCBI Official Symbol: IKBKAP
• NCBI Official Synonym Symbols: FD; DYS; ELP1; IKAP; IKI3; TOT1
• NCBI Protein Information: elongator complex protein 1; p150; IKK complex-associated protein; ikappaB kinase complex-associated protein; elongator acetyltransferase complex subunit 1
• UniProt Protein Name: Elongator complex protein 1
• UniProt Gene Name: IKBKAP
• UniProt Synonym Gene Names: ELP1; IKAP; ELP1; IKK complex-associated protein
• UniProt Entry Name: ELP1_HUMAN

NCBI Description

The protein encoded by this gene is a scaffold protein and a regulator for 3 different kinases involved in proinflammatory signaling. This encoded protein can bind NF-kappa-B-inducing kinase (NIK) and IKKs through separate domains and assemble them into an active kinase complex. Mutations in this gene have been associated with familial dysautonomia. [provided by RefSeq, Jul 2008]

Uniprot Description

IKBKAP: May act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK). Interacts preferentially with MAP3K14/NIK followed by IKK-alpha and IKK-beta. Component of the RNA polymerase II elongator complex (Elongator), which consists of IKBKAP/ELP1, STIP1/ELP2, ELP3, ELP4, and two yet unidentified proteins, p30 and p38. Elongator associates with the C-terminal domain (CTD) of Pol II largest subunit. Interacts with ELP3. Belongs to the ELP1/IKA1 family.

Protein type: Protein kinase, regulatory subunit; Transcription regulation

Chromosomal Location of Human Ortholog: 9q31

Cellular Component: transcription elongation factor complex; Elongator holoenzyme complex; cytoplasm; nucleolus; histone acetyltransferase complex

Molecular Function: protein binding; signal transducer activity; phosphorylase kinase regulator activity

Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; RNA elongation from RNA polymerase II promoter; immune response; protein complex assembly; signal transduction; regulation of protein kinase activity; protein amino acid phosphorylation; positive regulation of cell migration

Disease: Neuropathy, Hereditary Sensory And Autonomic, Type Iii