Rabbit anti-Human, Mouse PIKE Polyclonal Antibody | anti-AGAP2 antibody

PIKE Antibody

Cat. Num. AAA150273

• Gene Names: AGAP2; PIKE; GGAP2; CENTG1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: PIKE Antibody is Ion exchange chromatography purified.
• Synonyms: PIKE; Polyclonal Antibody; PIKE Antibody; GGAP2; CENTG1; KIAA0167; Arf-GAP with GTPase; ANK repeat and PH domain-containing protein 2; Centaurin-gamma-1; AGAP-2; ArfGAP with GTPase domain; ankyrin repeat and PH domain 2; anti-AGAP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Anti-PIKE will detect both PIKE-L and PIKE-A isoforms.
• Purity/Purification: PIKE Antibody is Ion exchange chromatography purified.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1192

• Applicable Applications for anti-AGAP2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: PIKE antibody can be used for detection of PIKE by Western blot at 0.5 to 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: PIKE antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human PIKE.
• Buffer: PIKE Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: PIKE antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PIKE in mouse brain cell lysates with PIKE antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL. )

Immunohistochemistry (IHC)

(Immunohistochemistry of PIKE in mouse brain tissue with PIKE antibody at 10 μg/mL.)

Related Product Information for anti-AGAP2 antibody

PIKE Antibody: Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity. PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1. The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes. In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 25989575
• NCBI GeneID: 116986
• UniProt Accession #: Q99490; O00578; Q548E0; Q8IWU3; A8K9F7
• Molecular Weight: 90,537 Da
• NCBI Official Full Name: PI 3-kinase enhancer long isoform
• NCBI Official Synonym Full Names: ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
• NCBI Official Symbol: AGAP2
• NCBI Official Synonym Symbols: PIKE; GGAP2; CENTG1
• NCBI Protein Information: arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2; cnt-g1; centaurin, gamma 1; phosphoinositide 3-kinase enhancer; phosphatidylinositol 3-kinase enhancer; phosphatidylinositol-3-kinase enhancer; GTP-binding and GTPase activating protein 2; Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2
• UniProt Protein Name: Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
• Protein Family: Arf-GAP with GTPase, ANK repeat and PH domain-containing protein
• UniProt Gene Name: AGAP2
• UniProt Synonym Gene Names: CENTG1; KIAA0167; AGAP-2; Cnt-g1; GGAP2; PIKE
• UniProt Entry Name: AGAP2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the centaurin gamma-like family. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. It is overexpressed in cancer cells, and promotes cancer cell invasion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

CENTG1: a member of the centaurin family of ADP-ribosylation factor directed GTPase-activating proteins (GAPs). A GAP for ARF1 and ARF5. Two alternatively-spliced isoforms have been described. Isoform 1 is brain-specific, and isoform 2 is ubiquitously expressed, with highest levels in brain and heart. Isoform 1 participates to the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. Regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. The PH domains of isoforms 1 and 2 bind phospholipids, but they differentially regulate subcellular location. The PH domain of isoform 1 directs the protein to the nucleus, but the PH domain of isoform 2 directs it to the cytosol.

Protein type: Motility/polarity/chemotaxis; GAPs; GAPs, ARF; Tumor suppressor

Chromosomal Location of Human Ortholog: 12q14.1

Cellular Component: membrane; mitochondrion; nucleolus; nucleus

Molecular Function: protein binding; GTP binding; zinc ion binding

Biological Process: axon guidance; protein transport; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; negative regulation of protein catabolic process; positive regulation of GTPase activity