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Western Blot (WB) (Figure. Western Blot; Sample: Recombinant THRb, Human.)

Rabbit anti-Human Thyroid Hormone Receptor Beta Antibody | anti-THRb antibody

Polyclonal Antibody to Thyroid Hormone Receptor Beta (THRb)

Gene Names
THRB; GRTH; PRTH; THR1; ERBA2; NR1A2; THRB1; THRB2; C-ERBA-2; C-ERBA-BETA
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Thyroid Hormone Receptor Beta; Polyclonal Antibody to Thyroid Hormone Receptor Beta (THRb); anti-THRb antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-THRb antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-2ug/mL;
Immunohistochemistry: 5-20ug/mL;
Immunocytochemistry: 5-20ug/mL;
Optimal working dilutions must be determined by end user.
Organism Species
Homo sapiens (Human)
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant THRb (Trp239~Glu457 (Accession # P10828)) expressed in E.coli (MBS2031485)
Cross Reactivity
Human
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 24 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Figure. Western Blot; Sample: Recombinant THRb, Human.)

Western Blot (WB) (Figure. Western Blot; Sample: Recombinant THRb, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human THRb Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human THRb Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
thyroid hormone receptor beta isoform a
NCBI Official Synonym Full Names
thyroid hormone receptor beta
NCBI Official Symbol
THRB
NCBI Official Synonym Symbols
GRTH; PRTH; THR1; ERBA2; NR1A2; THRB1; THRB2; C-ERBA-2; C-ERBA-BETA
NCBI Protein Information
thyroid hormone receptor beta
UniProt Protein Name
Thyroid hormone receptor beta
Protein Family
UniProt Gene Name
THRB
UniProt Synonym Gene Names
ERBA2; NR1A2; THR1

NCBI Description

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Mutations in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). Several alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.

Research Articles on THRb

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Product Notes

The THRb thrb (Catalog #AAA2025864) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Thyroid Hormone Receptor Beta (THRb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Thyroid Hormone Receptor Beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the THRb thrb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thyroid Hormone Receptor Beta, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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