Rabbit anti-Human Thyroid Hormone Receptor Beta Antibody | anti-THRb antibody

Polyclonal Antibody to Thyroid Hormone Receptor Beta (THRb)

Cat. Num. AAA2025864

• Gene Names: THRB; GRTH; PRTH; THR1; ERBA2; NR1A2; THRB1; THRB2; C-ERBA-2; C-ERBA-BETA
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Thyroid Hormone Receptor Beta; Polyclonal Antibody to Thyroid Hormone Receptor Beta (THRb); anti-THRb antibody

• Host: Rabbit
• Reactivity: Human
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 500ug/mL (varies by lot)

• Applicable Applications for anti-THRb antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: Western blotting: 0.5-2ug/mL;; Immunohistochemistry: 5-20ug/mL;; Immunocytochemistry: 5-20ug/mL;; Optimal working dilutions must be determined by end user.
• Organism Species: Homo sapiens (Human)
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Immunogen: Recombinant THRb (Trp239~Glu457 (Accession # P10828)) expressed in E.coli (MBS2031485)
• Cross Reactivity: Human
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles.; Store at 4ºC for frequent use.; Aliquot and store at -20ºC for 24 months.; ; Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Figure. Western Blot; Sample: Recombinant THRb, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human THRb Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

NCBI and Uniprot Product Information

• NCBI GI #: 40806162
• NCBI GeneID: 7068
• NCBI Accession #: NP_000452.2
• NCBI GenBank Nucleotide #: NM_000461.4
• UniProt Accession #: P10828; P37243; Q13986; Q3KP35; Q6WGL2; Q9UD41; B3KU79
• NCBI Official Full Name: thyroid hormone receptor beta isoform a
• NCBI Official Synonym Full Names: thyroid hormone receptor beta
• NCBI Official Symbol: THRB
• NCBI Official Synonym Symbols: GRTH; PRTH; THR1; ERBA2; NR1A2; THRB1; THRB2; C-ERBA-2; C-ERBA-BETA
• NCBI Protein Information: thyroid hormone receptor beta
• UniProt Protein Name: Thyroid hormone receptor beta
• Protein Family: Thyroid hormone receptor
• UniProt Gene Name: THRB
• UniProt Synonym Gene Names: ERBA2; NR1A2; THR1

NCBI Description

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Mutations in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). Several alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.