Rabbit anti-Rat, Human PIKE-L/S Polyclonal Antibody | anti-PIKE-L/S antibody

PIKE-L/S (Arf-GAP with GTPase, ANK Repeat and PH Domain-containing Protein 2, AGAP-2, Centaurin-gamma-1, Cnt-g1, GTP-binding and GTPase-activating Protein 2, GGAP2, Phosphatidylinositol-3-kinase Enhancer, AGAP2, CENTG1, KIAA0167) (APC)

Cat. Num. AAA6444755

• Gene Names: AGAP2; PIKE; GGAP2; CENTG1
• Reactivity: Rat, Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PIKE-L/S; Polyclonal Antibody; PIKE-L/S (Arf-GAP with GTPase; ANK Repeat and PH Domain-containing Protein 2; AGAP-2; Centaurin-gamma-1; Cnt-g1; GTP-binding and GTPase-activating Protein 2; GGAP2; Phosphatidylinositol-3-kinase Enhancer; AGAP2; CENTG1; KIAA0167) (APC); anti-PIKE-L/S antibody

• Host: Rabbit
• Reactivity: Rat, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat PIKE-L and PIKE-S doublet. Species Crossreactivity: human.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PIKE-L/S antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Fusion protein corresponding to aa268-384 of rat PIKE-L.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(PC-12 cell lysate was probed with anti-PIKE-L/S (0.5ug/ml). Arrow indicates PIKE-S (doublet at ~98kD).)

Related Product Information for anti-PIKE-L/S antibody

GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system.

Product Categories/Family for anti-PIKE-L/S antibody

Antibodies; Transport Proteins

References

1. PIKE-A is amplified in human cancers and prevents apoptosis by up-regulating Akt. Ahn, Jee-Yin, et al. (2004). Proc. Natl. Acad. Sci. U.S.A., 101: 6993-8 (2004). 2. PIKE GTPase: a novel mediator of phosphoinositide signaling. Ye, Keqiang and Snyder, Solomon H (2004). J. Cell. Sci., 117: 155-61 (2004). 3. Phospholipase C-gamma1: a phospholipase and guanine nucleotide exchange factor. Wang, Zhixiang and Moran, Michael F (2002). Mol. Interv., 2: 352-5, 338 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 25989575
• NCBI GeneID: 116986
• UniProt Accession #: Q99490; O00578; Q548E0; Q8IWU3; A8K9F7
• Molecular Weight: 90,537 Da
• NCBI Official Full Name: PI 3-kinase enhancer long isoform
• NCBI Official Synonym Full Names: ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
• NCBI Official Symbol: AGAP2
• NCBI Official Synonym Symbols: PIKE; GGAP2; CENTG1
• NCBI Protein Information: arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2; cnt-g1; centaurin, gamma 1; phosphoinositide 3-kinase enhancer; phosphatidylinositol 3-kinase enhancer; phosphatidylinositol-3-kinase enhancer; GTP-binding and GTPase activating protein 2
• UniProt Protein Name: Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
• UniProt Gene Name: AGAP2
• UniProt Synonym Gene Names: CENTG1; KIAA0167; AGAP-2; Cnt-g1; GGAP2; PIKE
• UniProt Entry Name: AGAP2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the centaurin gamma-like family. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. It is overexpressed in cancer cells, and promotes cancer cell invasion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

CENTG1: a member of the centaurin family of ADP-ribosylation factor directed GTPase-activating proteins (GAPs). A GAP for ARF1 and ARF5. Two alternatively-spliced isoforms have been described. Isoform 1 is brain-specific, and isoform 2 is ubiquitously expressed, with highest levels in brain and heart. Isoform 1 participates to the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. Regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. The PH domains of isoforms 1 and 2 bind phospholipids, but they differentially regulate subcellular location. The PH domain of isoform 1 directs the protein to the nucleus, but the PH domain of isoform 2 directs it to the cytosol.

Protein type: Motility/polarity/chemotaxis; GAPs; GAPs, ARF; Tumor suppressor

Chromosomal Location of Human Ortholog: 12q14.1

Cellular Component: membrane; mitochondrion; nucleolus; nucleus

Molecular Function: protein binding; GTP binding; zinc ion binding

Biological Process: axon guidance; protein transport; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; negative regulation of protein catabolic process; positive regulation of GTPase activity