Rabbit anti-Human, Mouse NGFR Polyclonal Antibody | anti-NGFR antibody

NGFR Antibody

Cat. Num. AAA150167

• Gene Names: NGFR; CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: NGFR Antibody is affinity chromatography purified via peptide column.
• Synonyms: NGFR; Polyclonal Antibody; NGFR Antibody; CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR; Tumor necrosis factor receptor superfamily member 16; NGF receptor; nerve growth factor receptor (TNFR superfamily; member 16); anti-NGFR antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: NGFR Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 427

• Applicable Applications for anti-NGFR antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: NGFR antibody can be used for the detection of NGFR by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: NGFR antibody was raised against purified recombinant human NGFR.
• Buffer: NGFR Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: NGFR antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of (A) 25 ng, (B) 50 ng, and (C) 100 ng of purified recombinant NGFR with NGFR antibody at 1 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of NGFR in A20 cells with NGFR antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of NGFR in HepG2 cells with NGFR antibody at 20 μg/mL.Green: NGFR Antibody (3593)Blue: DAPI staining)

Related Product Information for anti-NGFR antibody

NGFR Antibody: The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate numerous biological functions including cell proliferation, differentiation, and survival through regulating the activation of the transcription factor NF-κB and various mitogen-activated protein kinases. Nerve growth factor receptor (NGFR) was one of the earliest characterized members of this family. Also known as the low-affinity receptor p75NTR, this receptor is involved in several diverse functions such as apoptosis, neurite outgrowth during development, and myelination. Its ligands include NGF, brain-derived neurotrophic factor (BDNF), NT3, and NT4. NGFR can also associate with other NGF receptors such as Trk through the cytosolic and transmembrane domains and thus can function as a co-receptor that refines Trk affinity and specificity for neurotrophins. Finally, upon binding of various neurotrophins, NGFR associates with tumor necrosis factor receptor-6 (TRAF6), suggesting that it can potentially function as a signal transducer for NGF signals through NGFR.

NCBI and Uniprot Product Information

• NCBI GI #: 4505393
• NCBI GeneID: 4804
• NCBI Accession #: NP_002498
• NCBI GenBank Nucleotide #: NM_002507.3
• UniProt Accession #: P08138; B2R961; B4E096
• Molecular Weight: 35,767 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 16
• NCBI Official Synonym Full Names: nerve growth factor receptor
• NCBI Official Symbol: NGFR
• NCBI Official Synonym Symbols: CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 16; p75 ICD; NGF receptor; TNFR superfamily, member 16; low affinity nerve growth factor receptor; low affinity neurotrophin receptor p75NTR; low-affinity nerve growth factor receptor
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 16
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: NGFR
• UniProt Synonym Gene Names: TNFRSF16; NGF receptor
• UniProt Entry Name: TNR16_HUMAN

NCBI Description

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008]

Uniprot Description

NGFR: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. Interacts with KIDINS220 and NTRK1. Can form a ternary complex with NTRK1 and KIDINS220 and this complex is affected by the expression levels of KIDINS220. An increase in KIDINS220 expression leads to a decreased association of NGFR and NTRK1. Interacts with NTRK2; may regulate the ligand specificity of the NTRK2 receptor. Interacts with LINGO1.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 17q21-q22

Cellular Component: nucleoplasm; cell surface; integral to plasma membrane; plasma membrane; extracellular region; cytosol; endosome

Molecular Function: signal transducer activity; protein binding; transmembrane receptor activity; death receptor activity; ubiquitin protein ligase binding; nerve growth factor binding; receptor activity; Rab GTPase binding

Biological Process: detection of temperature stimulus; axon guidance; hair follicle morphogenesis; membrane protein intracellular domain proteolysis; central nervous system development; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; negative regulation of fibroblast growth factor receptor signaling pathway; positive regulation of odontogenesis of dentine-containing teeth; regulation of caspase activity; glucose homeostasis; regulation of axonogenesis; negative regulation of hair follicle development; negative regulation of cell cycle; intracellular protein transport; positive regulation of fibroblast proliferation; negative regulation of axonogenesis; positive regulation of axonogenesis; nerve development; circadian regulation of gene expression; negative regulation of apoptosis