Lactate Dehydrogenase A Active Protein | LDHA active protein

Recombinant Mouse Lactate Dehydrogenase A

Cat. Num. AAA146800

• Gene Names: Ldha; Ldh1; Ldhm; l7R2
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Lactate Dehydrogenase A; Recombinant Mouse Lactate Dehydrogenase A; LDHA Mouse; L-lactate dehydrogenase A chain; LDH-A; LDH muscle subunit; LDH-M; Lactate Dehydrogenase A Mouse Recombinant; LDHA active protein

• Host: Sf9, Baculovirus cells
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: Sterile filtered colorless solution.; LDHA protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
• Sequence: MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELALVDVMEDKLKG EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFIIPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGVHALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEVHKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGISDVVKVTLTP EEEARLKKSA DTLWGIQKEL QFLEHHHHHH
• Sequence Length: 332

• Biological Activity: Specific activity is > 250 units/mg, and is defined as the Amount of enzyme that convert 1.0 umole of pyruvate to L-lactate and per minute at pH 7.5 at 37C.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for LDHA active protein

Description: LDHA Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 340 amino acids (1-332a.a.) and having a molecular mass of 37.5kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). LDHA is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Introduction: LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA is localized primarily in muscle tissue and is part of the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. LDH1 is decreased in essential thrombocythemia. LDHA is induced through a non-genomic pathway of estrogen action. Reduction in LDH-A activity results in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential.

Product Categories/Family for LDHA active protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 6754524
• NCBI GeneID: 16828
• NCBI Accession #: NP_034829.1
• NCBI GenBank Nucleotide #: NM_010699.2
• UniProt Accession #: P06151
• Molecular Weight: 36,499 Da
• NCBI Official Full Name: L-lactate dehydrogenase A chain isoform 1
• NCBI Official Synonym Full Names: lactate dehydrogenase A
• NCBI Official Symbol: Ldha
• NCBI Official Synonym Symbols: Ldh1; Ldhm; l7R2
• NCBI Protein Information: L-lactate dehydrogenase A chain
• UniProt Protein Name: L-lactate dehydrogenase A chain
• Protein Family: L-lactate dehydrogenase
• UniProt Gene Name: Ldha
• UniProt Synonym Gene Names: Ldh-1; Ldh1; LDH-A; LDH-M

NCBI Description

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to hemolytic anemia and early postimplantation death in mice. Multiple transcript variants encoding different isoforms have been found for this gene. The mouse genome contains multiple pseudogenes of this gene. [provided by RefSeq, May 2013]

Uniprot Description

LDH-A: Homotetramer. Belongs to the LDH/MDH superfamily. LDH family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - cysteine and methionine; Apoptosis; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; EC 1.1.1.27; Oxidoreductase

Chromosomal Location of Human Ortholog: 7 B3|7 30.6 cM

Cellular Component: cell-cell adherens junction; cytosol; membrane; mitochondrion; nucleus

Molecular Function: L-lactate dehydrogenase activity; lactate dehydrogenase activity

Biological Process: cellular response to extracellular stimulus; glucose catabolic process to lactate via pyruvate; lactate biosynthetic process from pyruvate