Human Thyroid Stimulating Hormone ELISA Kit | TSH elisa kit

Human Thyroid Stimulating Hormone ELISA Kit

Cat. Num. AAA14634

• Gene Names: CGA; HCG; LHA; FSHA; GPHa; TSHA; GPHA1; CG-ALPHA
• Reactivity: Human
• Synonyms: Thyroid Stimulating Hormone; Human Thyroid Stimulating Hormone ELISA Kit; TSH elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human TSH and analogues was observed.
• Sequence Length: 116

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.625mU/L-20mU/L.
• Sensitivity: 0.1mU/L.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated TSH concentrations in Human serum, plasma and other body fluids. Using Purified Human TSH antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TSH and TSH antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for TSH elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated TSH concentrations in Human serum, plasma and other body fluids. Using Purified Human TSH antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TSH and TSH antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 546845
• NCBI GeneID: 1081
• UniProt Accession #: P01215
• Molecular Weight: 13,075 Da
• NCBI Official Full Name: thyroid-stimulating hormone alpha subunit
• NCBI Official Synonym Full Names: glycoprotein hormones, alpha polypeptide
• NCBI Official Symbol: CGA
• NCBI Official Synonym Symbols: HCG; LHA; FSHA; GPHa; TSHA; GPHA1; CG-ALPHA
• NCBI Protein Information: glycoprotein hormones alpha chain; FSH-alpha; LSH-alpha; TSH-alpha; lutropin alpha chain; follitropin alpha chain; thyrotropin alpha chain; choriogonadotropin alpha chain; luteinizing hormone alpha chain; chorionic gonadotrophin subunit alpha; thyroid-stimulating hormone alpha chain; follicle-stimulating hormone alpha chain; chorionic gonadotropin, alpha polypeptide; follicle-stimulating hormone alpha subunit; anterior pituitary glycoprotein hormones common subunit alpha
• UniProt Protein Name: Glycoprotein hormones alpha chain
• Protein Family: Temperature-sensitive hemagglutinin tsh autotransporter
• UniProt Gene Name: CGA
• UniProt Synonym Gene Names: CG-alpha; FSH-alpha; LSH-alpha; TSH-alpha
• UniProt Entry Name: GLHA_HUMAN

NCBI Description

The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

CGA: The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6q12-q21

Cellular Component: extracellular region

Molecular Function: protein binding; hormone activity

Biological Process: cellular protein metabolic process; cell-cell signaling; positive regulation of cell proliferation; peptide hormone processing; positive regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of cell migration