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Typical Testing Data/Standard Curve (for reference only)

Human Thyroid Stimulating Hormone (TSH) ELISA Kit | TSH elisa kit

Human Thyroid Stimulating Hormone (TSH) ELISA Kit

Gene Names
CGA; HCG; LHA; FSHA; GPHa; TSHA; GPHA1; CG-ALPHA
Reactivity
Human
Synonyms
Thyroid Stimulating Hormone (TSH); Human Thyroid Stimulating Hormone (TSH) ELISA Kit; TSH elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
No cross-reaction with other factors.
Sequence Length
116
Samples
Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant TSH concentration
Assay Type
Sandwich
Detection Range
20 mIU/L-0.312 mIU/L
Sensitivity
0.06 mIU/L.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TSH elisa kit
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Human TSH monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
References
New analytical application of antibody-based biosensor in estimation of thyroid-stimulating hormone in serum. Tanveer A Wani, Seema Zargar, Salma Majid Wakil, & Ibrahim A Darwish (2016)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
13,075 Da
NCBI Official Full Name
thyroid-stimulating hormone alpha subunit
NCBI Official Synonym Full Names
glycoprotein hormones, alpha polypeptide
NCBI Official Symbol
CGA
NCBI Official Synonym Symbols
HCG; LHA; FSHA; GPHa; TSHA; GPHA1; CG-ALPHA
NCBI Protein Information
glycoprotein hormones alpha chain; FSH-alpha; LSH-alpha; TSH-alpha; lutropin alpha chain; follitropin alpha chain; thyrotropin alpha chain; choriogonadotropin alpha chain; luteinizing hormone alpha chain; chorionic gonadotrophin subunit alpha; thyroid-stimulating hormone alpha chain; follicle-stimulating hormone alpha chain; chorionic gonadotropin, alpha polypeptide; follicle-stimulating hormone alpha subunit; anterior pituitary glycoprotein hormones common subunit alpha
UniProt Protein Name
Glycoprotein hormones alpha chain
UniProt Gene Name
CGA
UniProt Synonym Gene Names
CG-alpha; FSH-alpha; LSH-alpha; TSH-alpha
UniProt Entry Name
GLHA_HUMAN

NCBI Description

The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

CGA: The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6q12-q21

Cellular Component: extracellular region

Molecular Function: protein binding; hormone activity

Biological Process: cellular protein metabolic process; cell-cell signaling; positive regulation of cell proliferation; peptide hormone processing; positive regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of cell migration

Research Articles on TSH

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Product Notes

The Human TSH cga (Catalog #AAA264744) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA264744 ELISA Kit recognizes Human TSH. It is sometimes possible for the material contained within the vial of "Thyroid Stimulating Hormone (TSH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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