Negative elongation factor B (Cobra1) Recombinant Protein | Cobra1 recombinant protein

Recombinant Mouse Negative elongation factor B (Cobra1)

Cat. Num. AAA1454465

• Gene Names: Nelfb; Cobra1; Nelf-b; AB041607; AI663983; A730008L03Rik
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Negative elongation factor B (Cobra1); Recombinant Mouse Negative elongation factor B (Cobra1); Cobra1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-580, Full length protein
• Sequence: MFAGLQDLGVANGEDLKETLTNCTEPLKAIEQFQTENGVLLPSLQSALPFLDLHGTPRLEFHQSVFDELRDKLLERVSAIASEGKAEERYKKLEDLLEKSFSLVKMPSLQPVVMCVMKHLPKVPEKKLKLVMADKELYRACAVEVKRQIWQDNQALFGDEVSPLLKQYILEKESALFSTELSVLHNFFSPSPKTRRQGEVVQKLTQMVGKNVKLYDMVLQFLRTLFLRTRNVHYCTLRAELLMSLHDLDVSDICTVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLSLSTIRHLQELVSQETLPRDSPDLLLLLRLLALGQGAWDLIDSQVFKEPKMEAELITKFLPMLMSLVVDDFTFNVDQKLPAEEKASVTYPNTLPESFTKFLQEQRMACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFSDIFLHLLTGSLVLLADEFALEDFCSSLFDGFFLTASPRKENVHRHVLRLLLHLHARVAPSKLEALQKALEPTGQSGEAVKELYSQLGEKLEQLDHRKPSPTQAAETPALDLPLPSVPAPATL
• Sequence Length: 580

• Species: Mouse
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 862669347
• NCBI GeneID: 58202
• NCBI Accession #: NP_001297086.1
• NCBI GenBank Nucleotide #: NM_001310157.1
• UniProt Accession #: Q8C4Y3; Q99J41; Q9JJA5
• Molecular Weight: 65,636 Da
• NCBI Official Full Name: negative elongation factor B isoform 2
• NCBI Official Synonym Full Names: negative elongation factor complex member B
• NCBI Official Symbol: Nelfb
• NCBI Official Synonym Symbols: Cobra1; Nelf-b; AB041607; AI663983; A730008L03Rik
• NCBI Protein Information: negative elongation factor B
• UniProt Protein Name: Negative elongation factor B
• UniProt Gene Name: Nelfb
• UniProt Synonym Gene Names: Cobra1; NELF-B

NCBI Description

This gene encodes subunit B of a metazoan-specific, four-subunit protein complex that regulates promoter-proximal pausing of RNA polymerase II. RNA polymerase II pausing is thought to be important for coordination of gene transcription during embryonic development and stress responses. Consistently, disruption of this gene in mouse causes inner cell mass deficiency and embryonic lethality. In addition, this gene is required for maintenance of mouse embryonic stem cells by preventing expression of developmental genes. In adult mice, conditional deletion of this gene results in cardiomyopathy and impaired response to cardiac stress. Multiple protein isoforms are encoded through the use of a non-AUG (CUG) initiation codon and an alternative downstream AUG initiation codon. In addition, alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Binds RNA which may help to stabilize the NELF complex on nucleic acid. May be able to induce chromatin unfolding ().