Rabbit NELFB Polyclonal Antibody | anti-NELFB antibody

NELFB Antibody

Cat. Num. AAA9611932

• Gene Names: NELFB; COBRA1; NELF-B
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Dog, Chicken
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: NELFB; Polyclonal Antibody; NELFB Antibody; A730008L03Rik; AB041607; AI663983; Cofactor of BRCA1; DKFZp586B0519; KIAA1182; Negative elongation factor B; Negative elongation factor protein B; Nelf b; NELF-B; RGD1307832; RP13-122B23.3; RP23-132N23.4; anti-NELFB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Dog, Chicken
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NELFB Antibody detects endogenous levels of NELFB.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 580

• Applicable Applications for anti-NELFB antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human NELFB.
• Tissue Specificity: Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using NELFB Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-NELFB antibody

Description: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding.

Subunit Structure: The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE; the N-terminus of NELFB binds to the NELFA:NELFCD subcomplex. Interacts with the first BRCT repeat of BRCA1. Interacts with KIAA1191.

Similarity: Belongs to the NELF-B family.

NCBI and Uniprot Product Information

• NCBI GI #: 38372378
• NCBI GeneID: 25920
• UniProt Accession #: Q8WX92
• Molecular Weight: Observed: 66 kDa; Predicted: 66 kDa
• NCBI Official Full Name: Negative elongation factor B
• NCBI Official Synonym Full Names: negative elongation factor complex member B
• NCBI Official Symbol: NELFB
• NCBI Official Synonym Symbols: COBRA1; NELF-B
• NCBI Protein Information: negative elongation factor B
• UniProt Protein Name: Negative elongation factor B
• UniProt Gene Name: NELFB
• UniProt Synonym Gene Names: COBRA1; KIAA1182; NELF-B
• UniProt Entry Name: NELFB_HUMAN

NCBI Description

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]

Uniprot Description

COBRA1: a subunit of the multisubunit transcription elongation factor NELF (negative elongation factor). May be involved in transcriptional pausing of RNA polymerase II (RNAPII). Interacts with the first BRCT repeat of BRCA1.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression; negative regulation of transcription, DNA-dependent