Signal Recognition Particle Recombinant Protein | SRP54 recombinant protein
Recombinant Human Signal Recognition Particle 54kDa
Applications
Western Blot, ELISA
Purity
Greater than 80% as determined by SDS-PAGE.
Synonyms
Signal Recognition Particle; Recombinant Human Signal Recognition Particle 54kDa; SRP54 Human; Signal Recognition Particle 54kDa Human Recombinant; Signal Recognition Particle 54 kDa protein; SRP54 recombinant protein
Host
Sf9 Insect Cells
Purity/Purification
Greater than 80% as determined by SDS-PAGE.
Form/Format
SRP54 is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol.
Concentration
0.5-0.9 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. (varies by lot)
Sequence Length
455
Applicable Applications for SRP54 recombinant protein
Western Blot (WB), ELISA (EIA), Immunodot
Application Notes
Western blot with myositis sera or monoclonal anti-hexa-His-tag antibody.
Immunological Tests: Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels.
Immunological Tests: Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels.
Immunological Functions
Binds IgG-type human auto-antibodies.
Related Product Information for SRP54 recombinant protein
Description: SRP54 is a full-length cDNA coding for the human SRP54 protein having a molecular mass of 56,528 Dalton (pH 8.9). SRP54 protein is fused to a hexa-histidine purification tag.
Introduction: SRP54 is a 54-kDa subunit of the signal recognition particle (SRP), a cytoplasmic ribonucleoprotein complex which leads the translocation of newly synthesized secretory proteins from the polysome to the endoplasmic reticulum. SRP is composed of six polypeptides and a tRNA-like molecule known as 7SL RNA. SRP54 is a GTP-binding protein that directly binds the signal sequences of nascent secretory and membrane proteins. Anti-SRP autoantibodies take place in patients with an autoimmune chronic muscle inflammation called polymyositis. These auto-antibodies predominantly recognize the SRP54 subunit and in addition are able to immunoprecipitate several of the SRP subunits and the RNA component. Nearly 5% of myositis patients are positive for anti-SRP autoantibodies, increasing to 18% in the subgroup of Jo-1 autoantibody-negative patients. The classic 'anti-SRP syndrome' is a severe form of polymyositis in which the myositic inflammation is acute and aggressive onset, with common myalgias and cardiac involvement. Normally there is a poor reaction to therapy with a 5-year survival rate of about 25%.
Introduction: SRP54 is a 54-kDa subunit of the signal recognition particle (SRP), a cytoplasmic ribonucleoprotein complex which leads the translocation of newly synthesized secretory proteins from the polysome to the endoplasmic reticulum. SRP is composed of six polypeptides and a tRNA-like molecule known as 7SL RNA. SRP54 is a GTP-binding protein that directly binds the signal sequences of nascent secretory and membrane proteins. Anti-SRP autoantibodies take place in patients with an autoimmune chronic muscle inflammation called polymyositis. These auto-antibodies predominantly recognize the SRP54 subunit and in addition are able to immunoprecipitate several of the SRP subunits and the RNA component. Nearly 5% of myositis patients are positive for anti-SRP autoantibodies, increasing to 18% in the subgroup of Jo-1 autoantibody-negative patients. The classic 'anti-SRP syndrome' is a severe form of polymyositis in which the myositic inflammation is acute and aggressive onset, with common myalgias and cardiac involvement. Normally there is a poor reaction to therapy with a 5-year survival rate of about 25%.
Product Categories/Family for SRP54 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,369 Da
NCBI Official Full Name
signal recognition particle 54 kDa protein isoform 2
NCBI Official Synonym Full Names
signal recognition particle 54kDa
NCBI Official Symbol
SRP54
NCBI Protein Information
signal recognition particle 54 kDa protein; signal recognition particle 54kD
UniProt Protein Name
Signal recognition particle 54 kDa protein
Protein Family
UniProt Gene Name
SRP54
UniProt Synonym Gene Names
SRP54
UniProt Entry Name
SRP54_HUMAN
Uniprot Description
SRP54: Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein). Belongs to the GTP-binding SRP family. SRP54 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding
Chromosomal Location of Human Ortholog: 14q13.2
Cellular Component: cytoplasm; signal recognition particle, endoplasmic reticulum targeting; nucleolus; nuclear speck; nucleus; cytosol
Molecular Function: GTPase activity; ribonucleoprotein binding; GDP binding; GTP binding; drug binding; endoplasmic reticulum signal peptide binding; 7S RNA binding
Biological Process: response to drug; SRP-dependent cotranslational protein targeting to membrane, translocation; protein targeting to ER; SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translation; SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition; gene expression
Research Articles on SRP54
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Product Notes
The SRP54 srp54 (Catalog #AAA145095) is a Recombinant Protein produced from Sf9 Insect Cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Signal Recognition Particle can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunodot. Western blot with myositis sera or monoclonal anti-hexa-His-tag antibody. Immunological Tests: Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels. Researchers should empirically determine the suitability of the SRP54 srp54 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Signal Recognition Particle, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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