Chicken Signal Recognition Particle, 54kD Polyclonal Antibody | anti-SRP54 antibody

Signal Recognition Particle, 54kD (SRP54)

Cat. Num. AAA610209

• Gene Names: SRP54; SRP54
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Signal Recognition Particle; 54kD; Polyclonal Antibody; 54kD (SRP54); Anti -Signal Recognition Particle; anti-SRP54 antibody

• Host: Chicken
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes human Signal Recognition Particle, 54kD. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Glycerol free.

• Applicable Applications for anti-SRP54 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:500
• Immunogen: Synthetic peptide corresponding to aa300-504 of SRP54.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot Test: E coli-derived fusion protein as test antigen. Affinity-Purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.)

Related Product Information for anti-SRP54 antibody

The signal recognition particle (SRP) is an oligomeric complex that mediates targeting and insertion of the signal sequence of exported proteins into the membrane of the endoplasmic reticulum. SRP consists of a 7S RNA and six protein subunits. 54kD protein (SRP54) is one of these subunits. It is a GTP-binding protein that interacts with the signal sequence when it emerges from the ribosome. The 54kD subunit of the signal recognition particle has a two domain structure: the G-domain that binds GTP and the M-domain that binds the 7s RNA and also binds the signal sequence. The N-terminal 300 residues of SRP54 include the GTP-binding site (G-domain) and are evolutionary related to similar domains in other proteins.

Product Categories/Family for anti-SRP54 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 1256820
• NCBI GeneID: 6729
• UniProt Accession #: P61011; P13624; B2R759
• Molecular Weight: 55,705 Da
• NCBI Official Full Name: signal recognition particle
• NCBI Official Synonym Full Names: signal recognition particle 54kDa
• NCBI Official Symbol: SRP54
• NCBI Official Synonym Symbols: SRP54
• NCBI Protein Information: signal recognition particle 54 kDa protein; OTTHUMP00000178810
• UniProt Protein Name: Signal recognition particle 54 kDa protein
• Protein Family: Signal recognition particle
• UniProt Gene Name: SRP54
• UniProt Entry Name: SRP54_HUMAN

Uniprot Description

SRP54: Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein). Belongs to the GTP-binding SRP family. SRP54 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 14q13.2

Cellular Component: cytoplasm; signal recognition particle, endoplasmic reticulum targeting; nucleolus; nuclear speck; cytosol; nucleus

Molecular Function: GTPase activity; ribonucleoprotein binding; GDP binding; GTP binding; drug binding; endoplasmic reticulum signal peptide binding; 7S RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane, translocation; response to drug; SRP-dependent cotranslational protein targeting to membrane; protein targeting to ER; cellular protein metabolic process; translation; SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition; gene expression