Rabbit anti-Human SRP54 Polyclonal Antibody | anti-SRP54 antibody

SRP54, CT (SRP54, Signal recognition particle 54kD protein) (PE)

Cat. Num. AAA6351190

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SRP54; Polyclonal Antibody; CT (SRP54; Signal recognition particle 54kD protein) (PE); Signal recognition particle 54kD protein; anti-SRP54 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SRP54 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SRP54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345-374 amino acids from the C-terminal region of human SRP54.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SRP54 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the SRP54 antibody detected the SRP54 protein (arrow).)

Related Product Information for anti-SRP54 antibody

SRP54 binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).

Product Categories/Family for anti-SRP54 antibody

Antibodies; Signal Transduction

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Yoshida, M., et al. Mol. Pharmacol. 73(3):987-994(2008). Sakashita, E., et al. Mol. Cell. Biol. 24(3):1174-1187(2004). Kuglstatter, A., et al. Nat. Struct. Biol. 9(10):740-744(2002). Yin, J., et al. RNA 7(10):1389-1396(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 4507215
• NCBI GeneID: 6729
• NCBI Accession #: NP_003127.1; NP_001139754.1
• NCBI GenBank Nucleotide #: NM_003136.3
• UniProt Accession #: P61011; P13624; B2R759; B4DUW6
• Molecular Weight: 50,369 Da
• NCBI Official Full Name: signal recognition particle 54 kDa protein isoform 1
• NCBI Official Synonym Full Names: signal recognition particle 54kDa
• NCBI Official Symbol: SRP54
• NCBI Protein Information: signal recognition particle 54 kDa protein
• UniProt Protein Name: Signal recognition particle 54 kDa protein
• Protein Family: Signal recognition particle
• UniProt Gene Name: SRP54
• UniProt Synonym Gene Names: SRP54
• UniProt Entry Name: SRP54_HUMAN

Uniprot Description

SRP54: Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein). Belongs to the GTP-binding SRP family. SRP54 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 14q13.2

Cellular Component: cytoplasm; signal recognition particle, endoplasmic reticulum targeting; nucleolus; nuclear speck; nucleus; cytosol

Molecular Function: GTPase activity; ribonucleoprotein binding; GDP binding; GTP binding; drug binding; endoplasmic reticulum signal peptide binding; 7S RNA binding

Biological Process: response to drug; SRP-dependent cotranslational protein targeting to membrane, translocation; SRP-dependent cotranslational protein targeting to membrane; protein targeting to ER; cellular protein metabolic process; translation; SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition; gene expression