Protease Recombinant Protein | SCO2549 recombinant protein
Recombinant Protease
Gene Names
SCO2549; SCC77.16c.
Synonyms
Protease; Recombinant Protease; SCO2549 recombinant protein
Host
E Coli
Form/Format
Sterile filtered colorless solution.
Concentration
1000IU/1.43ml (varies by lot)
Sequence Length
1067
Cleavage Buffer
50mM Tris-HCl, pH-7.0 (at 25 degree C), 150mM NaCl, 1mM EDTA, 1mM dithiothreitol. Chill to 5 degree C prior to use.
Cleavage Conditions
For Cleavage of a Fusion Protein: During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. Optimal conditions for each fusion should be determined in pilot experiments. Digestion may be improved by adding TritonTM X-100, TweenTM 20, NonidetTM, or NP40 to a concentration of 0.01%. Concentrations of these detergents up to 1% do not inhibit PreScission Protease.
Unit Defenition
One unit will cleave ?90% of 100 ug of a test GST-fusion protein in Cleavage Buffer (50mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.0 at 25 degree C) at 5 degree C for 16 hours.
Preparation and Storage
Protease Recombinant although stable at 4 degree C for 1 week, should be stored desiccated below -18 degree C. Please prevent freeze thaw cycles.
Related Product Information for SCO2549 recombinant protein
Description: Protease Recombinant is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease. The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well.The Recombinant Protease is purified by proprietary chromatographic techniques.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
115,647 Da
NCBI Official Full Name
protease
NCBI Official Symbol
SCO2549
NCBI Official Synonym Symbols
SCC77.16c.
NCBI Protein Information
protease
UniProt Protein Name
Tricorn protease homolog 1
Protein Family
UniProt Gene Name
tri1
UniProt Entry Name
TRI1_STRCO
Uniprot Description
Degrades oligopeptides in a sequential manner.
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Product Notes
The SCO2549 tri1 (Catalog #AAA143200) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Protease, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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