Small Nuclear Ribonucleoprotein Recombinant Protein | SNRNP70 recombinant protein
Recombinant Human Small Nuclear Ribonucleoprotein 70kDa
Gene Names
SNRNP70; RPU1; Snp1; U1AP; U170K; U1RNP; RNPU1Z; SNRP70; U1-70K
Applications
Western Blot
Purity
Greater than 90% as determined by SDS-PAGE.
Synonyms
Small Nuclear Ribonucleoprotein; Recombinant Human Small Nuclear Ribonucleoprotein 70kDa; SNRNP70 Human; Small Nuclear Ribonucleoprotein 70kDa Human Recombinant; U1 small nuclear ribonucleoprotein 70 kDa; U1 snRNP 70 kDa; snRNP70; U1-70K; SNRNP70; RNPU1Z; RPU1; SNRP70; U1AP1; U1AP; U170K; U1RNP; SNRNP70 recombinant protein
Host
E Coli
Purity/Purification
Greater than 90% as determined by SDS-PAGE.
Form/Format
U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA and 0.02% SDS.
Sterile Filtered clear solution.
Sterile Filtered clear solution.
Concentration
0.3-0.6 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination. (varies by lot)
Sequence Length
428
Applicable Applications for SNRNP70 recombinant protein
Western Blot (WB)
Application Notes
Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera (Mixed Connective Tissue Disease).
Immunological Functions
1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.
Related Product Information for SNRNP70 recombinant protein
Description: Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein (lacking 66 internal amino acids outside the known epitope-containing areas) fused to a hexahistidine purification tag.The Calculated molecular weight is 44.8 kDa (U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight and the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis).
Introduction: Small nuclear ribonucleoprotein complexes (abbreviated as U-snRNP) are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus and consists of one small uridylate-rich RNA (U1 RNA) complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins (B/B', D1, D2, D3, E, F, G) form a core subparticle that is common to all U-snRNP complexes.Both the U1-specific proteins and the Sm core particle are targets of autoantibodies which classically have been called the RNP and RNP-Sm specificities, respectively. A clean distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity and specificity and is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease (MCTD) and 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells.
Introduction: Small nuclear ribonucleoprotein complexes (abbreviated as U-snRNP) are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus and consists of one small uridylate-rich RNA (U1 RNA) complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins (B/B', D1, D2, D3, E, F, G) form a core subparticle that is common to all U-snRNP complexes.Both the U1-specific proteins and the Sm core particle are targets of autoantibodies which classically have been called the RNP and RNP-Sm specificities, respectively. A clean distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity and specificity and is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease (MCTD) and 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells.
Product Categories/Family for SNRNP70 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,191 Da
NCBI Official Full Name
U1 small nuclear ribonucleoprotein 70 kDa isoform 2
NCBI Official Synonym Full Names
small nuclear ribonucleoprotein 70kDa (U1)
NCBI Official Symbol
SNRNP70
NCBI Official Synonym Symbols
RPU1; Snp1; U1AP; U170K; U1RNP; RNPU1Z; SNRP70; U1-70K
NCBI Protein Information
U1 small nuclear ribonucleoprotein 70 kDa; U1 snRNP 70 kDa
UniProt Protein Name
U1 small nuclear ribonucleoprotein 70 kDa
Protein Family
UniProt Gene Name
SNRNP70
UniProt Synonym Gene Names
RNPU1Z; RPU1; SNRP70; U1AP1; U1 snRNP 70 kDa; U1-70K; snRNP70
UniProt Entry Name
RU17_HUMAN
Uniprot Description
snRNP 70: mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. Participates in pre-mRNA splicing and exonic splicing complexes. A major ribonucleoprotein antigen recognized by the sera from patients with autoimmune diseases, such as systemic lupus erythematosus. Four alternatively spliced human isoforms have been described. The truncated isoforms (2-4) cannot bind U1-snRNA.
Protein type: RNA-binding; RNA splicing; Spliceosome
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: nucleoplasm; spliceosome; nuclear speck; snRNP U1; nucleus
Molecular Function: protein binding; RNA binding; nucleotide binding
Biological Process: nuclear mRNA splicing, via spliceosome; positive regulation of nuclear mRNA splicing, via spliceosome; regulation of RNA splicing; RNA splicing; gene expression; mRNA processing
Research Articles on SNRNP70
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Product Notes
The SNRNP70 snrnp70 (Catalog #AAA142999) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Small Nuclear Ribonucleoprotein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera (Mixed Connective Tissue Disease). Researchers should empirically determine the suitability of the SNRNP70 snrnp70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Small Nuclear Ribonucleoprotein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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