bPhosphoinositide 3-kinase alpha p110a/p85a Active Protein | bPI3Ka active protein
Recombinant bovine Phosphoinositide 3-kinase alpha p110a/p85a
Purity
Greater than 90.0% as determined by SDS-PAGE.
Synonyms
bPhosphoinositide 3-kinase alpha p110a/p85a; Recombinant bovine Phosphoinositide 3-kinase alpha p110a/p85a; PI3Ka Bovine; Phosphoinositide 3-kinase alpha p110a/p85a Bovine Recombinant; Phosphoinositide 3-kinase alpha p110a/p85a; PI3Ka; 2.7.1.137; Phosphatidylinositol 3-kinase; 1-phosphatidylinositol 3-kinase; PI3-kinase; PtdIns-3-kinase; Type I phosphatidylinositol kinase; Type III phosphoinositide 3-kinase; bPI3Ka active protein
Host
Sf9 Insect Cells
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE.
Form/Format
PI3Ka solution in 25mM HEPES, pH 8.0, 25mM NaCl, 2.5mM MgCl2 and 50% glycerol.
Sterile filtered liquid formualtion.
Sterile filtered liquid formualtion.
Unit Defenition
1 unit is defined as 1 picomole phosphate transferred to PIP2 per minute.
Activity
1,000 U/mg.
Preparation and Storage
PI3Ka although stable at 14 degree C for 1 week, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Related Product Information for bPI3Ka active protein
Description: The PI3Ka catalytic and regulatory subunits are coexpressed in Sf9 insect cells. Phosphoinositide 3-kinase alpha Bovine Recombinant is a glycosilated protein having a molecular weight as follows: p85a chain 83.5 kDa, p110 chain 124.3 kDa.
Introduction: PI3Ka plays a specific role in apoptosis in human colon cancer cells. Injection of neutralizing antibodies specific to PI3Ka into adenocarcinoma cells induced apoptosis, a response that was reverted by treating cells with caspase inhibitor. It was also shown that PI3Ka mediated phosphorylation of the p85a adapter reduces the lipid kinase activity of the heterodimer and this gives hints for PI3K-dependent signaling events not requiring production of 3'-phosphorylated phosphoinositides.PI3Ka is a key regulator of the initiation of keratinocyte differentiation. A decrease in PI3Ka activity results in a loss of keratinocyte adhesion to the extracellular membrane and the initiation of early phase differentiation.
Introduction: PI3Ka plays a specific role in apoptosis in human colon cancer cells. Injection of neutralizing antibodies specific to PI3Ka into adenocarcinoma cells induced apoptosis, a response that was reverted by treating cells with caspase inhibitor. It was also shown that PI3Ka mediated phosphorylation of the p85a adapter reduces the lipid kinase activity of the heterodimer and this gives hints for PI3K-dependent signaling events not requiring production of 3'-phosphorylated phosphoinositides.PI3Ka is a key regulator of the initiation of keratinocyte differentiation. A decrease in PI3Ka activity results in a loss of keratinocyte adhesion to the extracellular membrane and the initiation of early phase differentiation.
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Product Notes
The bPI3Ka (Catalog #AAA142821) is an Active Protein produced from Sf9 Insect Cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "bPhosphoinositide 3-kinase alpha p110a/p85a, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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