Granulocyte Macrophage-Colony Stimulating Factor Active Protein | rGM active protein
Recombinant Rat Granulocyte Macrophage-Colony Stimulating Factor
Gene Names
Csf2; Gmcsf; Gm-csf
Purity
Greater than 95.0% as determined by: (a)Analysis by RP-HPLC. (b)Analysis by SDS-PAGE.
Synonyms
Granulocyte Macrophage-Colony Stimulating Factor; Recombinant Rat Granulocyte Macrophage-Colony Stimulating Factor; GM CSF Rat; Granulocyte Macrophage-Colony Stimulating Factor Rat Recombinant; CSF-2; MGI-1GM; GM-CSF; Pluripoietin-alpha; Molgramostin; Sargramostim; rGM CSF; rGM active protein
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by: (a)Analysis by RP-HPLC. (b)Analysis by SDS-PAGE.
Form/Format
GM-CSF Rat was lyophilized with no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg
Sequence Length
144
Solubility
It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
The ED50 range = 0.001-0.01ng/ml corresponding to a specific activity of 100,000,000-1,000,000,000IU/mg, determined by the dose dependent proliferation of murine MC/9 cells.
Preparation and Storage
Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution GMCSF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Related Product Information for rGM active protein
Description: Granulocyte Macrophage Colony Stimulating Factor Rat Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14590.65 Dalton. GM-CSF Rat Recombinant is purified by proprietary chromatographic techniques.
Introduction: GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Introduction: GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Product Categories/Family for rGM active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,562 Da
NCBI Official Full Name
granulocyte-macrophage colony-stimulating factor
NCBI Official Synonym Full Names
colony stimulating factor 2 (granulocyte-macrophage)
NCBI Official Symbol
Csf2
NCBI Official Synonym Symbols
Gmcsf; Gm-csf
NCBI Protein Information
granulocyte-macrophage colony-stimulating factor; CSF
UniProt Protein Name
Granulocyte-macrophage colony-stimulating factor
UniProt Gene Name
Csf2
UniProt Synonym Gene Names
Csfgm; GM-CSF; CSF
UniProt Entry Name
CSF2_RAT
NCBI Description
plays a role in alveolar epithelial fluid transport [RGD, Feb 2006]
Uniprot Description
GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.
Protein type: Secreted, signal peptide; Secreted; Cytokine
Cellular Component: extracellular space
Molecular Function: growth factor activity; granulocyte macrophage colony-stimulating factor receptor binding; cytokine activity
Biological Process: negative regulation of cytolysis; epithelial fluid transport; embryonic placenta development; positive regulation of interleukin-23 production; myeloid dendritic cell differentiation; regulation of cell proliferation; epithelial cell proliferation; macrophage activation; positive regulation of tyrosine phosphorylation of Stat5 protein; regulation of gene expression; positive regulation of cell proliferation; immune response; positive regulation of macrophage differentiation; positive regulation of DNA replication
Research Articles on rGM
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Product Notes
The rGM csf2 (Catalog #AAA142271) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pr o-Thr-Arg. It is sometimes possible for the material contained within the vial of "Granulocyte Macrophage-Colony Stimulating Factor, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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