Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Testing Data (1,3 105 HEL cells were lysed (lysis buffer containing 1% Brij97 or triton) Migration SDS/page 5-15% gel under non-reducing conditions)

Mouse anti-Human CD63 Monoclonal Antibody | anti-CD63 antibody

Mouse Anti-Human CD63 Monoclonal Antibody, Azide Free Clone TS63

Gene Names
CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Purity
Ion exchange chromatography
Synonyms
CD63; Monoclonal Antibody; Mouse Anti-Human CD63 Monoclonal Antibody; Azide Free Clone TS63; anti-CD63 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
TS63
Specificity
Recognises the LIMP antigen, a 30-60 kDa (smear) protein
Purity/Purification
Ion exchange chromatography
Form/Format
Phosphate-buffered saline. Sterile-filtered through 0.22 um. Carrier and preservative free
Concentration
200ug / 200ul (varies by lot)
Applicable Applications for anti-CD63 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
Antigen
CD63
Hybridoma
Myeloma X63/AG8. x Balb/c spleen cells
Immunization
Jurkat and HEL cell lines
Conjugate
Azide free
Preparation and Storage
Stable at +2-8 degree C for 12 months. For longer storage, freeze aliquots

Testing Data

(1,3 105 HEL cells were lysed (lysis buffer containing 1% Brij97 or triton) Migration SDS/page 5-15% gel under non-reducing conditions)

Testing Data (1,3 105 HEL cells were lysed (lysis buffer containing 1% Brij97 or triton) Migration SDS/page 5-15% gel under non-reducing conditions)
References
• Charrin et.al Eur J Immunol 2003
• Charrin et.al FEBS lett 2002
• Charrin et.al. J Biol Chem 2001
• Sordat et.al Lab Invest 2002

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
967
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,637 Da
NCBI Official Full Name
CD63 antigen isoform D
NCBI Official Synonym Full Names
CD63 molecule
NCBI Official Symbol
CD63
NCBI Official Synonym Symbols
MLA1; ME491; LAMP-3; OMA81H; TSPAN30
NCBI Protein Information
CD63 antigen; tspan-30; granulophysin; tetraspanin-30; melanoma-associated antigen MLA1; CD63 antigen (melanoma 1 antigen); melanoma-associated antigen ME491; ocular melanoma-associated antigen; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3
UniProt Protein Name
CD63 antigen
Protein Family
UniProt Gene Name
CD63
UniProt Synonym Gene Names
MLA1; TSPAN30; LAMP-3; Tspan-30
UniProt Entry Name
CD63_HUMAN

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

Uniprot Description

Function: This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.

Subcellular location: Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Note: Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. Ref.3 Ref.13 Ref.15 Ref.17 Ref.18

Tissue specificity: Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.

Miscellaneous: Lack of expression of CD63 in platelets has been observed in a patient with Hermansky-Pudlak syndrome (HPS). Hermansky-Pudlak syndrome (HPS) is a genetically heterogeneous, rare, autosomal recessive disorder characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. This syndrome results from defects of diverse cytoplasmic organelles including melanosomes, platelet dense granules and lysosomes. Ceroid storage in the lungs is associated with pulmonary fibrosis, a common cause of premature death in individuals with HPS.

Sequence similarities: Belongs to the tetraspanin (TM4SF) family.

Research Articles on CD63

Similar Products

Product Notes

The CD63 cd63 (Catalog #AAA335647) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human CD63 Monoclonal Antibody, Azide Free Clone TS63 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD63 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the CD63 cd63 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD63, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.