Rabbit anti-Human DNAH2 Polyclonal Antibody | anti-DNAH2 antibody

DNAH2, NT (DNAH2, DNAHC2, DNHD3, KIAA1503, Dynein heavy chain 2, axonemal, Axonemal beta dynein heavy chain 2, Ciliary dynein heavy chain 2, Dynein heavy chain domain-containing protein 3) (Biotin)

Cat. Num. AAA6292566

• Gene Names: DNAH2; DNHD3; DNAHC2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DNAH2; Polyclonal Antibody; NT (DNAH2; DNAHC2; DNHD3; KIAA1503; Dynein heavy chain 2; axonemal; Axonemal beta dynein heavy chain 2; Ciliary dynein heavy chain 2; Dynein heavy chain domain-containing protein 3) (Biotin); Dynein heavy chain domain-containing protein 3; anti-DNAH2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-DNAH2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DNAH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 439-468 amino acids from the N-terminal region of human DNAH2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DNAH2 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DNAH2 antibody detected the DNAH2 protein (arrow).)

Related Product Information for anti-DNAH2 antibody

Dyneins are microtubule-associated motor protein complexes composed of several heavy, light, and intermediate chains. The axonemal dyneins, found in cilia and flagella, are components of the outer and inner dynein arms attached to the peripheral microtubule doublets. DNAH2 is an axonemal inner arm dynein heavy chain (Chapelin et al., 1997 [PubMed 9256245]).

Product Categories/Family for anti-DNAH2 antibody

Antibodies; KIAA; orf

References

Barrett, J.C., et al. Nat. Genet. (2009). In press: Wistow, G., et al. Mol. Vis. 8, 196-204 (2002): Chapelin, C., et al. FEBS Lett. 412(2):325-330(1997).

NCBI and Uniprot Product Information

• NCBI GI #: 75677365
• NCBI GeneID: 146754
• NCBI Accession #: NP_065928.2
• NCBI GenBank Nucleotide #: NM_020877.2
• UniProt Accession #: Q9P225; O15434; Q6PIH3; Q6ZR42; A8K992; B5MDX5
• Molecular Weight: 507,698 Da
• NCBI Official Full Name: dynein heavy chain 2, axonemal
• NCBI Official Synonym Full Names: dynein, axonemal, heavy chain 2
• NCBI Official Symbol: DNAH2
• NCBI Official Synonym Symbols: DNHD3; DNAHC2
• NCBI Protein Information: dynein heavy chain 2, axonemal; dynein heavy chain domain 3; ciliary dynein heavy chain 2; dynein, axonemal, heavy chain-2; axonemal beta dynein heavy chain 2; dynein, axonemal, heavy polypeptide 2; dynein heavy chain domain-containing protein 3
• UniProt Protein Name: Dynein heavy chain 2, axonemal
• Protein Family: Dynein
• UniProt Gene Name: DNAH2
• UniProt Synonym Gene Names: DNAHC2; DNHD3; KIAA1503
• UniProt Entry Name: DYH2_HUMAN

NCBI Description

Dyneins are microtubule-associated motor protein complexes composed of several heavy, light, and intermediate chains. The axonemal dyneins, found in cilia and flagella, are components of the outer and inner dynein arms attached to the peripheral microtubule doublets. DNAH2 is an axonemal inner arm dynein heavy chain (Chapelin et al., 1997 [PubMed 9256245]).[supplied by OMIM, Mar 2008]

Uniprot Description

Function: Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Involved in sperm motility; implicated in sperm flagellar assembly

By similarity.

Subunit structure: Consists of at least two heavy chains and a number of intermediate and light chains.

Subcellular location: Cytoplasm › cytoskeleton › cilium axoneme

Potential.

Tissue specificity: Expressed primarily in trachea and testis, 2 tissues containing axonemal structures. Also expressed in lung. Ref.5

Domain: Dynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function

By similarity.

Sequence similarities: Belongs to the dynein heavy chain family.Contains 5 TPR repeats.

RNA editing: Modified position: not applicable.Exon 13 included in isoform 3 is extensively edited in brain. Ref.6

Sequence caution: The sequence AK128517 differs from that shown. Reason: Frameshift at position 3963. The sequence BAA96027.2 differs from that shown. Reason: Erroneous initiation.