Rabbit SFPQ Polyclonal Antibody | anti-SFPQ antibody

SFPQ Polyclonal Antibody

Cat. Num. AAA127424

• Gene Names: SFPQ; PSF; POMP100; PPP1R140
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: SFPQ; Polyclonal Antibody; SFPQ Polyclonal Antibody; POMP100; PPP1R140; PSF; splicing factorproline and glutamine rich; anti-SFPQ antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Sequence Length: 707

• Applicable Applications for anti-SFPQ antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:1000; IHC 1:50 - 1:100; IF 1:50 - 1:100
• Immunogen: Recombinant fusion protein containing a sequencecorresponding to amino acids 578-707 of human SFPQ(NP_005057.1)
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using SFPQ antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using SFPQ antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using SFPQ antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human esophageal cancer using SFPQ antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using SFPQ antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse lung using SFPQ antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse brain using SFPQ antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse heart using SFPQ antibody at dilution of 1:100 (40x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells using SFPQ antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-SFPQ antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 1709851
• NCBI GeneID: 6421
• UniProt Accession #: P23246; P30808; Q5SZ71
• Molecular Weight: Calculated MW: 72kDa/76kDa; Observed MW: 115kDa
• NCBI Official Full Name: Splicing factor, proline- and glutamine-rich
• NCBI Official Synonym Full Names: splicing factor proline/glutamine-rich
• NCBI Official Symbol: SFPQ
• NCBI Official Synonym Symbols: PSF; POMP100; PPP1R140
• NCBI Protein Information: splicing factor, proline- and glutamine-rich; hPOMp100; 100 kDa DNA-pairing protein; PTB-associated splicing factor; PTB-associated-splicing factor; DNA-binding p52/p100 complex, 100 kDa subunit; protein phosphatase 1, regulatory subunit 140; polypyrimidine tract binding protein associated; polypyrimidine tract-binding protein-associated splicing factor; polypyrimidine tract-binding protein-associated-splicing factor; splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated); splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated)
• UniProt Protein Name: Splicing factor, proline- and glutamine-rich
• Protein Family: Splicing factor
• UniProt Gene Name: SFPQ
• UniProt Synonym Gene Names: PSF; hPOMp100; PSF; PTB-associated-splicing factor
• UniProt Entry Name: SFPQ_HUMAN

Uniprot Description

PSF: a proline-and glutamine-rich splicing factor. Essential pre-mRNA splicing factor required early in spliceosome formation. Seems to also bind DNA. The active complex is a heterotetramer of two 52 kDa and two 100 kDa subunits. Reported to be a PKC-alpha-binding protein in the cell nucleus. Two alternatively spliced isoforms have been described.

Protein type: RNA splicing; RNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: nucleoplasm; nuclear matrix; cytoplasm; nucleus; paraspeckles; chromatin

Molecular Function: protein binding; histone deacetylase binding; nucleotide binding

Biological Process: alternative nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; RNA splicing; rhythmic process; negative regulation of circadian rhythm; negative regulation of transcription from RNA polymerase II promoter; DNA repair; mRNA processing; negative regulation of transcription, DNA-dependent; regulation of circadian rhythm; DNA recombination