SDS-PAGE
DNA replication and repair protein RecF Recombinant Protein | recF recombinant protein
Recombinant Escherichia coli (strain K12) DNA replication and repair protein RecF
Gene Names
recF; ECK3692; JW3677; uvrF
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
DNA replication and repair protein RecF; Recombinant Escherichia coli (strain K12) DNA replication and repair protein RecF; recF recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
2-357aa; Full Length of Mature Protein
Sequence
SLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQRNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTFSFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISAEHVIDMSDENSKMFTVEKGKITD
Sequence Length
357
Production Note
Special Offer: The E Coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E Colihost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E Coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E Coli host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
SDS-PAGE
Related Product Information for recF recombinant protein
The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also ses to bind ATP.
References
Molecular analysis of the recF gene of Escherichia coli.Blanar M.A., Sandler S.J., Armengod M.-E., Ream L.W., Clark A.J.Proc. Natl. Acad. Sci. U.S.A. 81:4622-4626(1984)
DNA sequence and transcription of the region upstream of the E. coli gyrB gene.Adachi T., Mizuuchi K., Menzel R., Gellert M.Nucleic Acids Res. 12:6389-6395(1984)
DNA sequence and analysis of 136 kilobases of the Escherichia coli genome
organizational symmetry around the origin of replication.Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.Genomics 16:551-561(1993)
The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997)
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006)
Purification and preliminary characterization of the Escherichia coli K-12 recF protein.Griffin T.J. IV, Kolodner R.D.J. Bacteriol. 172:6291-6299(1990)
Sequence and complementation analysis of recF genes from Escherichia coli, Salmonella typhimurium, Pseudomonas putida and Bacillus subtilis
evidence for an essential phosphate binding loop.Sandler S.J., Chackerian B., Li J.T., Clark A.J.Nucleic Acids Res. 20:839-845(1992)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56.4 kDa
NCBI Official Full Name
gap repair protein
NCBI Official Symbol
recF
NCBI Official Synonym Symbols
ECK3692; JW3677; uvrF
NCBI Protein Information
gap repair protein
UniProt Protein Name
DNA replication and repair protein RecF
Protein Family
UniProt Gene Name
recF
UniProt Synonym Gene Names
uvrF
UniProt Entry Name
RECF_ECOLI
NCBI Description
[More information is available at EcoCyc: EG10828].
Uniprot Description
The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP.
Research Articles on recF
Similar Products
Product Notes
The recF recf (Catalog #AAA1081232) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 2-357aa; Full Length of Mature Protein. The amino acid sequence is listed below: SLTRLLIRDF RNIETADLAL SPGFNFLVGA NGSGKTSVLE AIYTLGHGRA FRSLQIGRVI RHEQEAFVLH GRLQGEERET AIGLTKDKQG DSKVRIDGTD GHKVAELAHL MPMQLITPEG FTLLNGGPKY RRAFLDWGCF HNEPGFFTAW SNLKRLLKQR NAALRQVTRY EQLRPWDKEL IPLAEQISTW RAEYSAGIAA DMADTCKQFL PEFSLTFSFQ RGWEKETEYA EVLERNFERD RQLTYTAHGP HKADLRIRAD GAPVEDTLSR GQLKLLMCAL RLAQGEFLTR ESGRRCLYLI DDFASELDDE RRGLLASRLK ATQSQVFVSA ISAEHVIDMS DENSKMFTVE KGKITD. It is sometimes possible for the material contained within the vial of "DNA replication and repair protein RecF, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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