Holliday junction ATP-dependent DNA helicase RuvA Recombinant Protein | ruvA recombinant protein

Recombinant Escherichia coli (strain K12) Holliday junction ATP-dependent DNA helicase RuvA

Cat. Num. AAA1102272

• Gene Names: ruvA; ECK1862; JW1850
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Holliday junction ATP-dependent DNA helicase RuvA; Recombinant Escherichia coli (strain K12) Holliday junction ATP-dependent DNA helicase RuvA; ruvA recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-203aa; Full Length
• Sequence: MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQEAIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAVEREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAEQEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL
• Sequence Length: 203

• Production Note: Special Offer: The E Coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E Colihost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E Coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E Coli host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for ruvA recombinant protein

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. Binds both single- and double-stranded DNA (dsDNA). Binds preferentially to supercoiled rather than to relaxed dsDNA.

References

Nucleotide sequencing of the ruv region of Escherichia coli K-12 reveals a LexA regulated operon encoding two genes.Benson F.E., Illing G.T., Sharples G.J., Lloyd R.G.Nucleic Acids Res. 16:1541-1549(1988) Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination.Shinagawa H., Makino K., Amemura M., Kimura S., Iwasaki H., Nakata A.J. Bacteriol. 170:4322-4329(1988) A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.DNA Res. 3:379-392(1996) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Molecular analysis of the Escherichia coli ruvC gene, which encodes a Holliday junction-specific endonuclease.Takahagi M., Iwasaki H., Nakata A., Shinagawa H.J. Bacteriol. 173:5747-5753(1991) RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase activity in vitro.Tsaneva I.R., Mueller B., West S.C.Proc. Natl. Acad. Sci. U.S.A. 90:1315-1319(1993) A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair.Babu M., Beloglazova N., Flick R., Graham C., Skarina T., Nocek B., Gagarinova A., Pogoutse O., Brown G., Binkowski A., Phanse S., Joachimiak A., Koonin E.V., Savchenko A., Emili A., Greenblatt J., Edwards A.M., Yakunin A.F.Mol. Microbiol. 79:484-502(2011) Crystal structure of DNA recombination protein RuvA and a model for its binding to the Holliday junction.Rafferty J.B., Sedelnikova S.E., Hargreaves D., Artymiuk P.J., Baker P.J., Sharples G.J., Mahdi A.A., Lloyd R.G., Rice D.W.Science 274:415-421(1996) Crystal structure of E Coli RuvA with bound DNA Holliday junction at 6-A resolution.Hargreaves D., Rice D.W., Sedelnikova S.E., Artymiuk P.J., Lloyd R.G., Rafferty J.B.Nat. Struct. Biol. 5:441-446(1998) Functional analyses of the domain structure in the Holliday junction binding protein RuvA.Nishino T., Ariyoshi M., Iwasaki H., Shinagawa H., Morikawa K.Structure 6:11-21(1998) Modulation of RuvB function by the mobile domain III of the Holliday junction recognition protein RuvA.Nishino T., Iwasaki H., Kataoka M., Ariyoshi M., Fujita T., Shinagawa H., Morikawa K.J. Mol. Biol. 298:407-416(2000) Processing of recombination intermediates by the RuvABC proteins.West S.C.Annu. Rev. Genet. 31:213-244(1997)

NCBI and Uniprot Product Information

• NCBI GI #: 16129814
• NCBI GeneID: 946369
• NCBI Accession #: NP_416375.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0A809; P08576
• Molecular Weight: 38.1 kDa
• NCBI Official Full Name: component of RuvABC resolvasome, regulatory subunit
• NCBI Official Symbol: ruvA
• NCBI Official Synonym Symbols: ECK1862; JW1850
• NCBI Protein Information: component of RuvABC resolvasome, regulatory subunit
• UniProt Protein Name: Holliday junction ATP-dependent DNA helicase RuvA
• Protein Family: Holliday junction ATP-dependent DNA helicase
• UniProt Gene Name: ruvA
• UniProt Entry Name: RUVA_ECOLI

NCBI Description

Binds Holliday junction as two tetramers (an octamer) and each tetrameric half recruits one RuvB hexameric helicase. [More information is available at EcoGene: EG10923]. RuvABC is an enzymatic complex which functions in recombination pathways to by binding to recombinational junctions and catalyzing strand cleavage and branch migration. [More information is available at EcoCyc: RUVA].

Uniprot Description

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. Binds both single- and double-stranded DNA (dsDNA). Binds preferentially to supercoiled rather than to relaxed dsDNA.