Intermediate capsid protein VP6 Recombinant Protein | VP6 recombinant protein

Recombinant Rotavirus A Intermediate capsid protein VP6

Cat. Num. AAA1191284

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Intermediate capsid protein VP6; Recombinant Rotavirus A Intermediate capsid protein VP6; VP6 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-397aa; Full Length
• Sequence: MDVLYSLSKTLKDARDKIVEGTLYSNVSDLIQQFNQMIITMNGNEFQTGGIGNLPIRNWNFDFGLLGTTLLNLDANYVETARNTIDYFVDFVDNVCMDEMVRESQRNGIAPQSDSLIKLSGIKFKRINFDNSSEYIENWNLQNRRQRTGFTFHKPNIFPYSASFTLNRSQPAHDNLMGTMWLNAGSEIQVAGFDYSCAINAPANTQQFEHIVQLRRVLTTATITLLPDAERFSFPRVITSADGATTWYFNPVILRPNNVEIEFLLNGQIINTYQARFGTIIARNFDTIRLSFQLMRPPNMTPAVAALFPNAQPFEHHATVGLTLRIESAVCESVLADASETMLANVTSVRQEYAIPVGPVFPPGMNWTDLITNYSPSREDNLQRVFTVASIRSMLVK
• Sequence Length: 397

• Production Note: Special Offer: The E Coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E Colihost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E Coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E Coli host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for VP6 recombinant protein

Intermediate capsid protein that self assbles to form an icosahedral capsid with a T=13 symmetry, which consists of 230 trimers of VP6, with channels at each of its five-fold vertices. This capsid constitutes the middle concentric layer of the viral mature particle. The innermost VP2 capsid and the intermediate VP6 capsid rain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP6 is required for the transcription activity of the DLP.

References

Cloning of bovine rotavirus (RF strain) nucleotide sequence of the gene coding for the major capsid protein.Cohen J., Lefevre F., Estes M.K., Bremont M.Virology 138:178-182(1984) Cohen J.Purification and characterization of bovine rotavirus cores.Bican P., Cohen J., Charpilienne A., Scherrer R.J. Virol. 43:1113-1117(1982) Identification of rotavirus VP6 residues located at the interface with VP2 that are essential for capsid assembly and transcriptase activity.Charpilienne A., Lepault J., Rey F.A., Cohen J.J. Virol. 76:7822-7831(2002) A zinc ion controls assembly and stability of the major capsid protein of rotavirus.Erk I., Huet J.-C., Duarte M., Duquerroy S., Rey F.A., Cohen J., Lepault J.J. Virol. 77:3595-3601(2003) Atomic structure of the major capsid protein of rotavirus implications for the architecture of the virion.Mathieu M., Petitpas I., Navaza J., Lepault J., Kohli E., Pothier P., Prasad B.V.V., Cohen J., Rey F.A.EMBO J. 20:1485-1497(2001)

NCBI and Uniprot Product Information

• NCBI GI #: 8247962
• UniProt Accession #: P04509
• Molecular Weight: 46.9 kDa
• NCBI Official Full Name: Intermediate capsid protein VP6
• UniProt Protein Name: Intermediate capsid protein VP6
• Protein Family: Intermediate capsid protein
• UniProt Entry Name: VP6_ROTRF

Uniprot Description

Intermediate capsid protein that self assembles to form an icosahedral capsid with a T=13 symmetry, which consists of 230 trimers of VP6, with channels at each of its five-fold vertices. This capsid constitutes the middle concentric layer of the viral mature particle. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nascent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP6 is required for the transcription activity of the DLP.