ZNRF1 recombinant protein

Recombinant Human ZNRF1 Protein

Cat. Num. AAA8250051

• Gene Names: ZNRF1; NIN283
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: ZNRF1; Recombinant Human ZNRF1 Protein; NIN283; E3 ubiquitin-protein ligase ZNRF1; Nerve injury-induced gene 283 protein; Zinc/RING finger protein 1; ZNRF1 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 muM filtered solution of 20mM Tris, 0.15M NaCl, 1mM EDTA, 10% Glycerol, pH 8.0.
• Sequence: MGSSHHHHHH SSGLVPRGSH MPASRGTGDS ERAPGGGGSA SDSTYAHGNG YQETGGGHHR DGMLYLGSRA SLADALPLHI APRWFSSHSG FKCPICSKSV ASDEMEMHFI MCLSKPRLSY NDDVLTLEHH HHHH
• Sequence Length: 227

• Applicable Applications for ZNRF1 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for ZNRF1 recombinant protein

Recombinant Human ZNRF1 Protein is produced by our E Coli expression system and the target gene encoding Pro74-Thr178 is expressed with a 6His tag at the N-terminus, 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 14150005
• NCBI GeneID: 84937
• NCBI Accession #: NP_115644.1
• NCBI GenBank Nucleotide #: NM_032268.4
• UniProt Accession #: Q8ND25; Q9H083; D3DUJ9
• Molecular Weight: 29,777 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase ZNRF1
• NCBI Official Synonym Full Names: zinc and ring finger 1
• NCBI Official Symbol: ZNRF1
• NCBI Official Synonym Symbols: NIN283
• NCBI Protein Information: E3 ubiquitin-protein ligase ZNRF1
• UniProt Protein Name: E3 ubiquitin-protein ligase ZNRF1
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: ZNRF1
• UniProt Synonym Gene Names: NIN283
• UniProt Entry Name: ZNRF1_HUMAN

NCBI Description

This gene encodes an E3 ubiquitin-protein ligase that plays a role in neural-cell differentiation. Overexpression of this gene causes neurite-like elongation. The encoded protein contains both a zinc finger and a RING finger motif and is localized in the endosome/lysosome compartment, indicating that it may be involved in ubiquitin-mediated protein modification, and in synaptic vessicle membranes in neurons. [provided by RefSeq, Feb 2012]

Uniprot Description

ZNRF1: E3 ubiquitin-protein ligase that mediates the ubiquitination of AKT1 and GLUL, thereby playing a role in neuron cells differentiation. Plays a role in the establishment and maintenance of neuronal transmission and plasticity. Regulates Schwann cells differentiation by mediating ubiquitination of GLUL. Promotes neurodegeneration by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of AKT1 in axons: degradation of AKT1 prevents AKT1-mediated phosphorylation of GSK3B, leading to GSK3B activation and phosphorylation of DPYSL2/CRMP2 followed by destabilization of microtubule assembly in axons (Probable). 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: cytosol; endosome; membrane

Molecular Function: protein binding; ubiquitin-protein ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination