Mouse Zinc finger protein 143 ELISA Kit | ZNF143 elisa kit
Mouse Zinc finger protein 143 (ZNF143) ELISA Kit
Gene Names
Zfp143; SBF; Staf; Zfp79; pHZ-1; KRAB14; Znf143; AA959806; AU015869; Zfp80-rs1; D7Ertd805e
Reactivity
Mouse
Synonyms
Zinc finger protein 143; Mouse Zinc finger protein 143 (ZNF143) ELISA Kit; ZNF143 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse ZNF143. No significant cross-reactivity or interference between Mouse ZNF143 and analogues was observed.
Sequence Length
637
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for ZNF143 elisa kit
Transcriptional regulatory proteins containing tandemly repeated zinc finger domains are thought to be involved in both normal and abnormal cellular proliferation and differentiation. One abundant class of such transcriptional regulators resembles the Drosophila Kruppel segmentation gene product due to the presence of repeated Cys2-His2 (C2H2) zinc finger domains that are connected by conserved sequences, called H/C links. Like Staf, both ZNF76 and ZNF143 contain 7 zinc fingers and an activation domain with the mRNA and snRNA activation regions. Both proteins bound Staf-responsive elements in vitro. Chimeric ZNF76 and ZNF143 proteins activated a pol II mRNA promoter and snRNA pol II and pol III promoters in Xenopus oocytes.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZNF143 in samples. An antibody specific for ZNF143 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZNF143 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZNF143 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZNF143 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZNF143 in samples. An antibody specific for ZNF143 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZNF143 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZNF143 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZNF143 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,030 Da
NCBI Official Full Name
zinc finger protein 143
NCBI Official Synonym Full Names
zinc finger protein 143
NCBI Official Symbol
Zfp143
NCBI Official Synonym Symbols
SBF; Staf; Zfp79; pHZ-1; KRAB14; Znf143; AA959806; AU015869; Zfp80-rs1; D7Ertd805e
NCBI Protein Information
zinc finger protein 143
UniProt Protein Name
Zinc finger protein 143
Protein Family
UniProt Gene Name
Znf143
UniProt Synonym Gene Names
Staf; Zfp143; Zfp-143; mStaf
Uniprot Description
ZNF143: a transcriptional activator that binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Interacts with p73. Involved in cisplatin resistance through the transcriptional regulation of DNA repair genes. Transcriptional tergets include BUB1B, TFAM and transaldolase. Association with CHD8 contributes to efficient U6 RNA polymerase III transcription.
Protein type: C2H2-type zinc finger protein; Transcription factor
Chromosomal Location of Human Ortholog: 7 E3|7 57.7 cM
Cellular Component: nucleus
Molecular Function: chromatin binding; DNA binding; metal ion binding; nucleic acid binding; protein binding
Biological Process: positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; transcription, DNA-dependent
Research Articles on ZNF143
Similar Products
Product Notes
The Mouse ZNF143 znf143 (Catalog #AAA282627) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282627 ELISA Kit recognizes Mouse ZNF143. It is sometimes possible for the material contained within the vial of "Zinc finger protein 143, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
