Mouse Zinc finger MIZ domain-containing protein 1 ELISA Kit | ZMIZ1 elisa kit
Mouse Zinc finger MIZ domain-containing protein 1 (ZMIZ1) ELISA Kit
Gene Names
Zmiz1; Rai17; Zimp10; Gm10397; BC065120; E330020C23; I920194n01
Reactivity
Mouse
Synonyms
Zinc finger MIZ domain-containing protein 1; Mouse Zinc finger MIZ domain-containing protein 1 (ZMIZ1) ELISA Kit; ZMIZ1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse ZMIZ1. No significant cross-reactivity or interference between Mouse ZMIZ1 and analogues was observed.
Sequence Length
1067
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for ZMIZ1 elisa kit
ZMIZ1 encodes a member of the PIAS (protein inhibitor of activated STAT) family of proteins. The encoded protein regulates the activity of various transcription factors, including the androgen receptor, Smad3/4, and p53. The encoded protein may also play a role in sumoylation. A translocation between this locus on chromosome 10 and the protein tyrosine kinase ABL1 locus on chromosome 9 has been associated with acute lymphoblastic leukemia.
The deduced 1,067-amino acid protein has a calculated molecular mass of 123 kD. RAI17 contains an MSX-interacting zinc finger (MIZ) domain, a nuclear localization signal sequence, and 2 proline-rich regions. In vitro transcription and translation resulted in a protein with an apparent molecular mass of 130 kD.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZMIZ1 in samples. An antibody specific for ZMIZ1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZMIZ1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZMIZ1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZMIZ1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
The deduced 1,067-amino acid protein has a calculated molecular mass of 123 kD. RAI17 contains an MSX-interacting zinc finger (MIZ) domain, a nuclear localization signal sequence, and 2 proline-rich regions. In vitro transcription and translation resulted in a protein with an apparent molecular mass of 130 kD.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZMIZ1 in samples. An antibody specific for ZMIZ1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZMIZ1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZMIZ1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZMIZ1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
115,199 Da
NCBI Official Full Name
zinc finger MIZ domain-containing protein 1 isoform 2
NCBI Official Synonym Full Names
zinc finger, MIZ-type containing 1
NCBI Official Symbol
Zmiz1
NCBI Official Synonym Symbols
Rai17; Zimp10; Gm10397; BC065120; E330020C23; I920194n01
NCBI Protein Information
zinc finger MIZ domain-containing protein 1
UniProt Protein Name
Zinc finger MIZ domain-containing protein 1
Protein Family
UniProt Gene Name
Zmiz1
UniProt Synonym Gene Names
Rai17; Zimp10
Uniprot Description
RAI17: Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 14|14 A3
Cellular Component: cytoplasm; intracellular membrane-bound organelle; nucleoplasm; nucleus
Molecular Function: metal ion binding; protein binding; zinc ion binding
Biological Process: artery morphogenesis; cell aging; developmental growth; heart morphogenesis; in utero embryonic development; positive regulation of fibroblast proliferation; positive regulation of Notch signaling pathway; positive regulation of T cell differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; transcription, DNA-dependent; vasculogenesis; vitellogenesis
Research Articles on ZMIZ1
1. Ectopic expression of Zmiz1 induces cutaneous squamous cell malignancies in a mouse model of cancer.
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Product Notes
The Mouse ZMIZ1 zmiz1 (Catalog #AAA282337) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA282337 ELISA Kit recognizes Mouse ZMIZ1. It is sometimes possible for the material contained within the vial of "Zinc finger MIZ domain-containing protein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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