Rabbit anti-Human, Mouse MGST1 Polyclonal Antibody | anti-MGST1 antibody

MGST1 (Microsomal Glutathione S-transferase 1, Microsomal GST-1, Microsomal GST-I, GST12, MGST, MGC14525) (HRP)

Cat. Num. AAA6385405

• Gene Names: MGST1; MGST; GST12; MGST-I
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MGST1; Polyclonal Antibody; MGST1 (Microsomal Glutathione S-transferase 1; Microsomal GST-1; Microsomal GST-I; GST12; MGST; MGC14525) (HRP); anti-MGST1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MGST1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MGST1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human MGST1, aa1-155 (NP_064696.1).
• Immunogen Sequence: MVDLTQVMDDEVFMAFASYATIILSKMMLMSTATAFYRLTRKVFANPEDCVAFGKGENAKKYLRTDDRVERVRRAHLNDLENIIPFLGIGLLYSLSGPDPSTAILHFRLFVGARIYHTIAYLTPLPQPNRALSFFVGYGVTLSMAYRLLKSKLYL
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MGST1 rabbit polyclonal antibody. Western Blot analysis of MGST1 expression in human liver.)

Western Blot (WB)

(MGST1 rabbit polyclonal antibody. Western Blot analysis of MGST1 expression in mouse kidney.)

Western Blot (WB)

(MGST1 rabbit polyclonal antibody. Western Blot analysis of MGST1 expression in HepG2.)

Western Blot (WB)

(Western Blot analysis of MGST1 expression in transfected 293T cell line by MGST1 polyclonal antibody. Lane 1: MGST1 transfected lysate (17.6kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-MGST1 antibody

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.

Product Categories/Family for anti-MGST1 antibody

Antibodies; GST

NCBI and Uniprot Product Information

• NCBI GI #: 9945306
• NCBI GeneID: 4257
• NCBI Accession #: NP_064696.1
• NCBI GenBank Nucleotide #: NM_020300.3
• UniProt Accession #: P10620; A8K533; G5EA53
• Molecular Weight: 9,984 Da
• NCBI Official Full Name: microsomal glutathione S-transferase 1 isoform a
• NCBI Official Synonym Full Names: microsomal glutathione S-transferase 1
• NCBI Official Symbol: MGST1
• NCBI Official Synonym Symbols: MGST; GST12; MGST-I
• NCBI Protein Information: microsomal glutathione S-transferase 1; microsomal GST-1; microsomal GST-I; glutathione S-transferase 12
• UniProt Protein Name: Microsomal glutathione S-transferase 1
• Protein Family: Microsomal glutathione S-transferase
• UniProt Gene Name: MGST1
• UniProt Synonym Gene Names: GST12; MGST; Microsomal GST-1
• UniProt Entry Name: MGST1_HUMAN

NCBI Description

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Several transcript variants, some non-protein coding and some protein coding, have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

MGST1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity. Belongs to the MAPEG family.

Protein type: EC 2.5.1.18; Mitochondrial; Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione; Membrane protein, multi-pass; Transferase; Membrane protein, integral; Xenobiotic Metabolism - drug metabolism - cytochrome P450

Chromosomal Location of Human Ortholog: 12p12.3-p12.1

Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; mitochondrial outer membrane; mitochondrion; apical part of cell; endoplasmic reticulum; mitochondrial inner membrane; integral to membrane; nucleus

Molecular Function: protein binding; protein homodimerization activity; glutathione transferase activity; glutathione binding; glutathione peroxidase activity

Biological Process: response to drug; glutathione metabolic process; Leydig cell differentiation; xenobiotic metabolic process; response to lipopolysaccharide; response to organic nitrogen