Human Zinc finger homeobox protein 3 ELISA Kit | ZFHX3 elisa kit

Human Zinc finger homeobox protein 3 ELISA Kit

Cat. Num. AAA760989

• Gene Names: ZFHX3; ATBT; ATBF1; ZNF927
• Reactivity: Human
• Synonyms: Zinc finger homeobox protein 3; Human Zinc finger homeobox protein 3 ELISA Kit; ZFHX3/Zinc finger homeobox protein 3/Zinc finger homeodomain protein 3/ZFH-3/Alpha-fetoprotein enhancer-binding protein/AT motif-binding factor/AT-binding transcription factor 1; ZFHX3 elisa kit

• Reactivity: Human
• Sequence Length: 3703

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ZFHX3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ZFHX3antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ZFHX3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ZFHX3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ZFHX3can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 118498345
• NCBI GeneID: 463
• NCBI Accession #: NP_008816.3
• NCBI GenBank Nucleotide #: NM_006885.3
• Molecular Weight: 306,664 Da
• NCBI Official Full Name: zinc finger homeobox protein 3 isoform A
• NCBI Official Synonym Full Names: zinc finger homeobox 3
• NCBI Official Symbol: ZFHX3
• NCBI Official Synonym Symbols: ATBT; ATBF1; ZNF927
• NCBI Protein Information: zinc finger homeobox protein 3
• UniProt Protein Name: Zinc finger homeobox protein 3
• Protein Family: Zinc finger homeobox protein
• UniProt Gene Name: ZFHX3
• UniProt Synonym Gene Names: ATBF1; ZFH-3
• UniProt Entry Name: ZFHX3_HUMAN

NCBI Description

This gene encodes a transcription factor with multiple homeodomains and zinc finger motifs, and regulates myogenic and neuronal differentiation. The encoded protein suppresses expression of the alpha-fetoprotein gene by binding to an AT-rich enhancer motif. The protein has also been shown to negatively regulate c-Myb, and transactivate the cell cycle inhibitor cyclin-dependent kinase inhibitor 1A (also known as p21CIP1). This gene is reported to function as a tumor suppressor in several cancers, and sequence variants of this gene are also associated with atrial fibrillation. Multiple transcript variants expressed from alternate promoters and encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

ATBF1: Transcriptional repressor. It inhibits the enhancer element of the AFP gene by binding to its AT-rich core sequence. Regulator of myoblasts differentiation through the binding to the AT-rich sequence of MYF6 promoter and promoter repression. Down- regulates the MUC5AC promoter in gastric cancer. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Cell development/differentiation; C2H2-type zinc finger protein; Mitochondrial; Transcription factor

Chromosomal Location of Human Ortholog: 16q22.3

Cellular Component: cytoplasm; intracellular membrane-bound organelle; mitochondrion; nuclear body; nucleolus; nucleoplasm; nucleus; transcription factor complex

Molecular Function: enzyme binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity; zinc ion binding

Biological Process: brain development; cell cycle arrest; circadian regulation of gene expression; muscle development; negative regulation of myoblast differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of myoblast differentiation; positive regulation of transcription, DNA-dependent; regulation of neuron differentiation; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter

Disease: Prostate Cancer