Mouse Zinc finger CCHC domain-containing protein 7 ELISA Kit | ZCCHC7 elisa kit

Mouse Zinc finger CCHC domain-containing protein 7 (ZCCHC7) ELISA Kit

Cat. Num. AAA282480

• Gene Names: Zcchc7; D4Wsu132e; 4930572I07Rik
• Reactivity: Mouse
• Synonyms: Zinc finger CCHC domain-containing protein 7; Mouse Zinc finger CCHC domain-containing protein 7 (ZCCHC7) ELISA Kit; ZCCHC7 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse ZCCHC7. No significant cross-reactivity or interference between Mouse ZCCHC7 and analogues was observed.
• Sequence Length: 543

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ZCCHC7 elisa kit

ZCCHC7 contains 4 CCHC-type zinc fingers and it has two isoforms. Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. Some of these domains bind zinc, but many do not; instead binding other metals such as iron, or no metal at all.
This entry represents the CysCysHisCys (CCHC) type zinc finger domains, and have the sequence: C-X2-C-X4-H-X4-C. where X can be any amino acid, and number indicates the number of residues. These 18 residues CCHC zinc finger domains are mainly found in the nucleocapsid protein of retroviruses. It is required for viral genome packaging and for early infection process. It is also found in eukaryotic proteins involved in RNA binding or single-stranded DNA binding.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZCCHC7 in samples. An antibody specific for ZCCHC7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZCCHC7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZCCHC7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZCCHC7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 169646700
• NCBI GeneID: 319885
• NCBI Accession #: NP_613056.2
• NCBI GenBank Nucleotide #: NM_138590.4
• UniProt Accession #: B1AX39; Q3TZN5; Q8C915; Q9CYZ9
• Molecular Weight: 26,226 Da
• NCBI Official Full Name: zinc finger CCHC domain-containing protein 7
• NCBI Official Synonym Full Names: zinc finger, CCHC domain containing 7
• NCBI Official Symbol: Zcchc7
• NCBI Official Synonym Symbols: D4Wsu132e; 4930572I07Rik
• NCBI Protein Information: zinc finger CCHC domain-containing protein 7
• UniProt Protein Name: Zinc finger CCHC domain-containing protein 7
• Protein Family: Zinc finger CCHC domain-containing protein
• UniProt Gene Name: Zcchc7
• UniProt Synonym Gene Names: D4Wsu132e

Uniprot Description

ZCCHC7: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 4 B1|4 23.57 cM

Cellular Component: cytosol; nucleolus; nucleus

Molecular Function: metal ion binding; nucleic acid binding; RNA binding; zinc ion binding