CTDSP1 recombinant protein

CTDSP1, 1-260aa, Human, His tag, E Coli

Cat. Num. AAA203810

• Gene Names: CTDSP1; NIF3; SCP1; NLIIF; NLI-IF
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: CTDSP1; 1-260aa; Human; His tag; E Coli; Carboxy-terminal domain RNA polymerase II polypeptide A phosphatase 1; Carboxy-terminal domain; RNA polymerase II; polypeptide A phosphatase 1; NLIIF; SCP1; NIF3; CTDSP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MDSSAVITQI SKEEARGPLR GKGDQKSAAS QKPRSRGILH SLFCCVCRDD GEALPAHSGA PLLVEENGAI PKTPVQYLLP EAKAQDSDKI CVVIDLDETL VHSSFKPVNN ADFIIPVEID GVVHQVYVLK RPHVDEFLQR MGELFECVLF TASLAKYADP VADLLDKWGA FRARLFRESC VFHRGNYVKD LSRLGRDLRR VLILDNSPAS YVFHPDNAVP VASWFDNMSD TELHDLLPFF EQLSRVDDVY SVLRQPRPGS
• Sequence Length: 260

• Applicable Applications for CTDSP1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CTDSP1 recombinant protein

Carboxy-terminal domain, RNA polymerase II, polypeptide A phosphatase 1, also known as CTDSP1, is a class 2C phosphatase with activity dependent on the conserved DxD motif. CTDSP1 preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Also it negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recombinant human CTDSP1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for CTDSP1 recombinant protein

Signal Transduction

References

Maiello B., et al. (2001) Front Biosci. 6: 1358-1368.; Palancade B., et al. (2004) J Mol Biol. 335:415-424.

NCBI and Uniprot Product Information

• NCBI GI #: 32813443
• NCBI GeneID: 58190
• NCBI Accession #: NP_872580
• NCBI GenBank Nucleotide #: NM_182642.2
• UniProt Accession #: Q9GZU7; Q7Z5Q3; Q7Z5Q4; C9IYG0
• Molecular Weight: 31.2 kDa (280aa), confirmed by MALDI-TOF
• NCBI Official Full Name: carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 isoform 2
• NCBI Official Synonym Full Names: CTD small phosphatase 1
• NCBI Official Symbol: CTDSP1
• NCBI Official Synonym Symbols: NIF3; SCP1; NLIIF; NLI-IF
• NCBI Protein Information: carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
• UniProt Protein Name: Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
• Protein Family: Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase
• UniProt Gene Name: CTDSP1
• UniProt Synonym Gene Names: NIF3; NLIIF; SCP1; NLI-IF; NLI-interacting factor 3; SCP1; Small CTD phosphatase 1

NCBI Description

This gene encodes a member of the small C-terminal domain phosphatase (SCP) family of nuclear phosphatases. These proteins play a role in transcriptional regulation through specific dephosphorylation of phosphoserine 5 within tandem heptapeptide repeats of the C-terminal domain of RNA polymerase II. The encoded protein plays a role in neuronal gene silencing in non-neuronal cells, and may also inhibit osteoblast differentiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.