Mouse 14-3-3 protein zeta/delta ELISA Kit | YWHAZ elisa kit

Mouse 14-3-3 protein zeta/delta (YWHAZ) ELISA Kit

Cat. Num. AAA2606985

• Gene Names: YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta
• Reactivity: Mouse
• Synonyms: 14-3-3 protein zeta/delta; Mouse 14-3-3 protein zeta/delta (YWHAZ) ELISA Kit; YWHAZ elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.
• Sequence Length: 245

• Samples: Mouse Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Vtn Concentration
• Assay Type: Sandwich
• Detection Range: 200 ng/ml-3.12 ng/ml
• Sensitivity: 0.6 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for YWHAZ elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse VTN monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 208973238
• NCBI GeneID: 7534
• NCBI Accession #: NP_001129171.1
• NCBI GenBank Nucleotide #: NM_001135699.1
• Molecular Weight: 19,333 Da
• NCBI Official Full Name: 14-3-3 protein zeta/delta
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
• NCBI Official Symbol: YWHAZ
• NCBI Official Synonym Symbols: HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta
• NCBI Protein Information: 14-3-3 protein zeta/delta
• UniProt Protein Name: 14-3-3 protein zeta/delta
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAZ
• UniProt Synonym Gene Names: KCIP-1
• UniProt Entry Name: 1433Z_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

14-3-3 zeta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Phosphorylation apparently disrupts homodimerization.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: cytoplasm; cytoplasmic vesicle membrane; cytosol; extracellular space; focal adhesion; melanosome; mitochondrion; nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; protein domain specific binding; protein kinase binding; transcription factor binding; ubiquitin protein ligase binding

Biological Process: apoptosis; blood coagulation; establishment of Golgi localization; gene expression; negative regulation of apoptosis; platelet activation; programmed cell death; protein targeting; regulation of mRNA stability; signal transduction; small GTPase mediated signal transduction; transcription initiation from RNA polymerase II promoter