Mouse anti-Human Thymidylate Synthase Monoclonal Antibody | anti-TS antibody

Thymidylate Synthase (TS) (FITC)

Cat. Num. AAA6243405

• Gene Names: TYMS; TS; TMS; HST422
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Thymidylate Synthase; Monoclonal Antibody; Thymidylate Synthase (TS) (FITC); anti-TS antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2Q466
• Specificity: Recognizes a 36kD protein, Thymidylate Synthase (TS). Species Crossreactivity: Human.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-TS antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Immunogen: Recombinant protein corresponding to Thymidylate Synthase.
• Conjugate: FITC
• Preparation and Storage: Store at -20 degree C.

Testing Data

(Thymidylate Synthase staining of human colon cancer using the T5155-14 monoclonal antibody)

Product Categories/Family for anti-TS antibody

Antibodies; Enzymes; Synthase

References

1. Johnston, P.G., et al. Cancer Res. (1991) 51: 6668. 2. Johnston, P.G., et al. Cancer Res. (1992) 52: 4306. 3. Johnston, P.G., et al. Biochem. Pharmacol. (1993) 45: 2483. 4. Johnston, P.G., et al. J. Clin. Oncol. (1994) 12: 2640. 5. Johnston, P.G., et al. Cancer Res. (1995) 55: 1407. 6. Johnston, P.G., et al. J. NCI (1997) 89: 308. 7. Pestalozzi, B.C., et al. J. Clin. Oncol. (1997) 15: 1923.

NCBI and Uniprot Product Information

• NCBI GI #: 136611
• NCBI GeneID: 7298
• UniProt Accession #: P04818; Q8WYK3; Q8WYK4
• Molecular Weight: Calculated MW: 26kDa/31kDa/35kDa; Observed MW: 36kDa
• NCBI Official Full Name: Thymidylate synthase
• NCBI Official Synonym Full Names: thymidylate synthetase
• NCBI Official Symbol: TYMS
• NCBI Official Synonym Symbols: TS; TMS; HST422
• NCBI Protein Information: thymidylate synthase; TSase
• UniProt Protein Name: Thymidylate synthase
• Protein Family: Thymidylate synthase
• UniProt Gene Name: TYMS
• UniProt Synonym Gene Names: TS; TS; TSase
• UniProt Entry Name: TYSY_HUMAN

NCBI Description

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq, Jul 2008]

Uniprot Description

TYMS: an enzyme in the thymidylate synthase family. Polymorphisms serve as prognostic markers for determining modality of treatment in certain cancers. Genotyping helps predict toxicity to 5-FU-based chemotherapy in patients with colorectal cancer and the response to platinum-based chemotherapy in advanced NSCLC. A common polymorphism is a determinant of red blood cell folate and homocysteine concentrations. Homodimer.

Protein type: Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - one carbon pool by folate; DNA repair, damage; EC 2.1.1.45; Mitochondrial; Methyltransferase

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; nucleolus; nucleus; cytosol

Molecular Function: mRNA binding; protein homodimerization activity; nucleotide binding; drug binding; cofactor binding; thymidylate synthase activity; folic acid binding

Biological Process: immortalization of host cell by virus; methylation; tetrahydrofolate metabolic process; circadian rhythm; uracil metabolic process; developmental growth; response to organophosphorus; response to toxin; response to glucocorticoid stimulus; dTTP biosynthetic process; response to vitamin A; pyrimidine base metabolic process; response to folic acid; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; aging; response to drug; organ regeneration; nucleobase, nucleoside and nucleotide metabolic process; dTMP biosynthetic process; deoxyribonucleoside monophosphate biosynthetic process; G1/S-specific transcription in mitotic cell cycle; response to ethanol; response to cytokine stimulus; cartilage development; pyrimidine nucleoside biosynthetic process; dUMP metabolic process; mitotic cell cycle; response to progesterone stimulus; G1/S transition of mitotic cell cycle