Chicken 14-3-3 protein zeta/delta ELISA Kit | YWHAZ elisa kit

Chicken 14-3-3 protein zeta/delta (YWHAZ) ELISA Kit

Cat. Num. AAA285779

• Gene Names: YWHAZ; 14-3-3zeta
• Reactivity: Chicken
• Synonyms: 14-3-3 protein zeta/delta; Chicken 14-3-3 protein zeta/delta (YWHAZ) ELISA Kit; 14-3-3-zeta; KCIP-1; MGC111427; MGC126532; MGC138156; 14-3-3 protein/cytosolic phospholipase A2|14-3-3 zeta|OTTHUMP00000165851|OTTHUMP00000165858|phospholipase A2|protein kinase C inhibitor protein-; YWHAZ elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chicken YWHAZ. No significant cross-reactivity or interference between Chicken YWHAZ and analogues was observed.
• Sequence Length: 245

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for YWHAZ elisa kit

YWHAz belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs.
The deduced 245-amino acid protein shares high sequence homology with other 14-3-3 subtypes. The zeta mRNA was widely expressed in various gray matter brain regions, including the neocortex, hippocampus, caudate-putamen, thalamus, cerebellar cortex, and several brain stem nuclei. A human protein with phospholipase A2 activity was shown to be the zeta subtype of the 14-3-3 protein

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate YWHAZ in samples. An antibody specific for YWHAZ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYWHAZ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAZ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAZ bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 71897035
• NCBI GeneID: 425619
• NCBI Accession #: NP_001026514.1
• NCBI GenBank Nucleotide #: NM_001031343.1
• UniProt Accession #: Q5ZKC9
• Molecular Weight: 27,775 Da
• NCBI Official Full Name: 14-3-3 protein zeta
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
• NCBI Official Symbol: YWHAZ
• NCBI Official Synonym Symbols: 14-3-3zeta
• NCBI Protein Information: 14-3-3 protein zeta
• UniProt Protein Name: 14-3-3 protein zeta
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAZ

Uniprot Description

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner ().