14-3-3 zeta recombinant protein

14-3-3 zeta recombinant protein

Cat. Num. AAA515276

• Gene Names: YWHAZ; YWHAD; KCIP-1; 14-3-3-zeta
• Applications: Western Blot
• Synonyms: 14-3-3 zeta; 14-3-3 zeta recombinant protein; YWHAZ; KCIP-1; MGC111427; MGC126532; MGC138156; 14-3-3zeta Protein

• Host: E Coli
• Form/Format: 50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 3003

• Applicable Applications for 14-3-3 zeta recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: E.coli
• Source Note: Recombinant full-length human tag-free 14-3-3zeta was expressed in E. coli cells.
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for 14-3-3 zeta recombinant protein

Recombinant full-length human 14-3-3zeta was expressed in E. coli cells using an N-terminal GST tag.

Scientific Background: 14-3-3ζ (also known as tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide) is a member of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3ζ protein plays a key role in cancer biology by being an important regulator of major cellular processes such as proliferation, differentiation, senescence and apoptosis (1). 14-3-3ζ protein has been shown to interact with the IRS1 protein, suggesting a role for this protein in regulating insulin sensitivity by interrupting the association between the insulin receptor and IRS1 (2).

Product Categories/Family for 14-3-3 zeta recombinant protein

Cell Stress & Chaperone Proteins; Signaling Proteins - Cell Stress & Chaperone Proteins

References

1. Li, Z. et al: Down-regulation of 14-3-3zeta suppresses anchorage-independent growth of lung cancer cells through anoikis activation. Proc Natl Acad Sci U S A. 2008; 105(1):162-7.
2. Niemantsverdriet, M. et al: Cellular functions of 14-3-3 zeta in apoptosis and cell adhesion emphasize its oncogenic character. Oncogene. 2008;27(9):1315-9. 2007.

NCBI and Uniprot Product Information

• NCBI GI #: 208973235
• NCBI GeneID: 7534
• NCBI Accession #: NP_003397.1
• NCBI GenBank Nucleotide #: NM_003406
• UniProt Accession #: P63104; P29213; P29312; Q32P43; Q5XJ08; Q6GPI2; Q6IN74; Q6NUR9; Q6P3U9; Q86V33; A8K1N0; B7Z465
• Molecular Weight: ~55 kDa
• NCBI Official Full Name: Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (YWHAZ), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
• NCBI Official Symbol: YWHAZ
• NCBI Official Synonym Symbols: YWHAD; KCIP-1; 14-3-3-zeta
• NCBI Protein Information: 14-3-3 protein zeta/delta; 14-3-3 zeta; 14-3-3 delta; phospholipase A2; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; 14-3-3 protein/cytosolic phospholipase A2; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide
• UniProt Protein Name: 14-3-3 protein zeta/delta
• UniProt Gene Name: YWHAZ
• UniProt Synonym Gene Names: KCIP-1
• UniProt Entry Name: 1433Z_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Ref.9 Ref.14 Ref.15 Ref.21 Ref.23

Subunit structure: Interacts with CDK16 and BSPRY

By similarity. Interacts with WEE1 (C-terminal). Interacts with SAMSN1

By similarity. Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm

By similarity. Interacts with Thr-phosphorylated ITGB2

By similarity. Interacts with BCL2L11

By similarity. Homodimer. Heterodimerizes with YWHAE. Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58. Interacts with FOXO4, NOXA1, SSH1 and ARHGEF2. Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form). Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with TP53; the interaction enhances p53 transcriptional activity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with PKA-phosphorylated AANAT; the interaction modulates AANAT enzymatic activity by increasing affinity for arylalkylamines and acetyl-CoA and protecting the enzyme from dephosphorylation and proteasomal degradation. It may also prevent thiol-dependent inactivation. Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization. Interacts with GAB2 and TLK2. Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.20 Ref.21 Ref.23 Ref.25 Ref.26 Ref.27

Subcellular location: Cytoplasm. Melanosome. Note: Located to stage I to stage IV melanosomes. Ref.24

Post-translational modification: The delta, brain-specific form differs from the zeta form in being phosphorylated

By similarity. Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232; inhibits binding of RAF1. Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. Ref.9 Ref.12 Ref.13 Ref.15 Ref.19 Ref.20 Ref.23

Sequence similarities: Belongs to the 14-3-3 family.

Caution: Was originally (Ref.1) thought to have phospholipase A2 activity.

Sequence caution: The sequence AAH51814.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence AAH73141.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.