14-3-3 eta/YWHAH Recombinant Protein | YWHAH recombinant protein

14-3-3 eta/YWHAH Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8120520

• Gene Names: YWHAH; YWHA1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: 14-3-3 eta/YWHAH; 14-3-3 eta/YWHAH Protein; Human; Recombinant (GST Tag); Human 14-3-3 eta/YWHAH Protein (GST Tag); YWHA1 Protein; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; eta; YWHAH recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 0.15M NaCl, 20mM GST, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly2-Asn246

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the human YWHAH (Q04917)(Gly 2-Asn 246) was fused with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for YWHAH recombinant protein

Background: YWHAH, the gene encoding the 14-3-3eta isoform, is highly expressed in retinal ganglion cells (RGC). YWHAH is a positional and functional candidate gene for both schizophrenia and bipolar disorder (BP). It is located on chromosome 22q12.3, a region that has been implicated by linkage studies in both BP and schizophrenia.

Product Categories/Family for YWHAH recombinant protein

phospho-Serine/phospho-Threonine Binding Proteins Adaptor Proteins Apoptosis Adaptor Proteins; Death Receptor Signaling

References

Wakui H, et al. (1997) Interaction of the ligand-activated glucocorticoid receptor with the 14-3-3 eta protein. J Biol Chem. 272(13): 8153-6.Toyooka K, et al. (2002) Isolation and structure of the mouse 14-3-3 eta chain gene and the distribution of 14-3-3 eta mRNA in the mouse brain. Brain Res Mol Brain Res. 100(1-2): 13-20.Kim YS, et al. (2005) Role of 14-3-3 eta as a positive regulator of the glucocorticoid receptor transcriptional activation. Endocrinology. 146(7): 3133-40.Grover D, et al. (2009) Family-based association of YWHAH in psychotic bipolar disorder. Am J Med Genet B Neuropsychiatr Genet. 150B(7): 977-83.

NCBI and Uniprot Product Information

• NCBI GI #: 4507951
• NCBI GeneID: 7533
• NCBI Accession #: NP_003396.1
• NCBI GenBank Nucleotide #: NM_003405.3
• UniProt Accession #: Q04917
• Molecular Weight: 28,219 Da
• NCBI Official Full Name: 14-3-3 protein eta
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
• NCBI Official Symbol: YWHAH
• NCBI Official Synonym Symbols: YWHA1
• NCBI Protein Information: 14-3-3 protein eta; 14-3-3 eta
• UniProt Protein Name: 14-3-3 protein eta
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAH
• UniProt Synonym Gene Names: YWHA1
• UniProt Entry Name: 1433F_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009]

Uniprot Description

14-3-3 eta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: cytoplasmic vesicle membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: protein domain specific binding; insulin-like growth factor receptor binding; protein binding; enzyme binding; sodium channel regulator activity; protein heterodimerization activity; actin binding; glucocorticoid receptor binding

Biological Process: intracellular protein transport; substantia nigra development; apoptosis; regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; glucocorticoid receptor signaling pathway; regulation of synaptic plasticity; glucocorticoid catabolic process; negative regulation of dendrite morphogenesis; regulation of sodium ion transport