Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide ELISA Kit | YWHAH elisa kit

Human 14-3-3 protein eta, YWHAH ELISA Kit

Cat. Num. AAA922335

• Gene Names: YWHAH; YWHA1
• Reactivity: Human
• Synonyms: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; eta polypeptide; Human 14-3-3 protein eta; YWHAH ELISA Kit; Human 14-3-3 protein eta (YWHAH) ELISA kit; LL22NC03-44A4.1; YWHA1; 14-3-3 eta; YWHAH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human YWHAH. No significant cross-reactivity or interference between human YWHAH and analogues was observed.
• Sequence Length: 246

• Samples: Serum, plasma, tissue homogenates.
• Assay Type: Sandwich
• Detection Range: 0.625 ng/ml -40 ng/ml.
• Sensitivity: 0.156 ng /ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for YWHAH elisa kit

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for YWHAH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YWHAH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAH bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4507951
• NCBI GeneID: 7533
• NCBI Accession #: NP_003396.1
• NCBI GenBank Nucleotide #: NM_003405.3
• UniProt Accession #: Q04917
• Molecular Weight: 28,219 Da
• NCBI Official Full Name: 14-3-3 protein eta
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
• NCBI Official Symbol: YWHAH
• NCBI Official Synonym Symbols: YWHA1
• NCBI Protein Information: 14-3-3 protein eta; 14-3-3 eta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
• UniProt Protein Name: 14-3-3 protein eta
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAH
• UniProt Synonym Gene Names: YWHA1
• UniProt Entry Name: 1433F_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009]

Uniprot Description

14-3-3 eta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Nuclear receptor co-regulator; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: cytoplasmic vesicle membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: protein domain specific binding; insulin-like growth factor receptor binding; protein binding; enzyme binding; sodium channel regulator activity; protein heterodimerization activity; actin binding; glucocorticoid receptor binding

Biological Process: intracellular protein transport; substantia nigra development; regulation of neuron differentiation; apoptosis; glucocorticoid receptor signaling pathway; positive regulation of transcription, DNA-dependent; regulation of synaptic plasticity; glucocorticoid catabolic process; negative regulation of dendrite morphogenesis; regulation of sodium ion transport