Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide ELISA Kit | YWHAG elisa kit

Human 14-3-3 protein gamma, YWHAG ELISA Kit

Cat. Num. AAA929301

• Gene Names: YWHAG; PPP1R170; 14-3-3GAMMA
• Reactivity: Human
• Synonyms: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; gamma polypeptide; Human 14-3-3 protein gamma; YWHAG ELISA Kit; Human 14-3-3 protein gamma (YWHAG) ELISA kit; 14-3-3GAMMA; 14-3-3 gamma; YWHAG elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human YWHAG. No significant cross-reactivity or interference between human YWHAG and analogues was observed.
• Sequence Length: 247

• Samples: Serum, plasma, tissue homogenates.
• Assay Type: Sandwich
• Detection Range: 0.625 ng/ml -40 ng/ml.
• Sensitivity: 0.156 ng /ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for YWHAG elisa kit

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for YWHAG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YWHAG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAG bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 21464101
• NCBI GeneID: 7532
• NCBI Accession #: NP_036611.2
• NCBI GenBank Nucleotide #: NM_012479.3
• UniProt Accession #: P61981; O70457; P35214; Q6FH52; Q9UDP2; Q9UN99
• Molecular Weight: 28,303 Da
• NCBI Official Full Name: 14-3-3 protein gamma
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma
• NCBI Official Symbol: YWHAG
• NCBI Official Synonym Symbols: PPP1R170; 14-3-3GAMMA
• NCBI Protein Information: 14-3-3 protein gamma; KCIP-1; 14-3-3 gamma; protein kinase C inhibitor protein 1; protein phosphatase 1, regulatory subunit 170; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
• UniProt Protein Name: 14-3-3 protein gamma
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAG
• UniProt Synonym Gene Names: KCIP-1
• UniProt Entry Name: 1433G_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]

Uniprot Description

14-3-3 gamma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Homodimer. Interacts with SAMSN1. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT. Highly expressed in brain, skeletal muscle, and heart. Belongs to the 14-3-3 family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: cytoplasmic vesicle membrane; focal adhesion; membrane; cytosol

Molecular Function: protein domain specific binding; protein kinase C inhibitor activity; insulin-like growth factor receptor binding; protein binding; protein kinase C binding; receptor tyrosine kinase binding

Biological Process: cellular response to insulin stimulus; regulation of neuron differentiation; apoptosis; organelle organization and biogenesis; regulation of signal transduction; negative regulation of protein kinase activity; regulation of synaptic plasticity; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein targeting