14-3-3 epsilon / YWHAE recombinant protein

Recombinant Human 14-3-3 epsilon / YWHAE Protein

Cat. Num. AAA2546051

• Gene Names: YWHAE; MDS; HEL2; MDCR; KCIP-1; 14-3-3E
• Purity: > 96 % as determined by SDS-PAGE
• Synonyms: 14-3-3 epsilon / YWHAE; Recombinant Human 14-3-3 epsilon / YWHAE Protein; 14-3-3E; FLJ45465; FLJ53559; KCIP-1; MDCR; MDS; YWHAE; 14-3-3 epsilon / YWHAE recombinant protein

• Host: E Coli
• Purity/Purification: > 96 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.25mM DTT, 25% glycerol, 0.5mM GSH, pH 7.5
• Sequence Length: 255

• Application Notes: The recombinant human YWHAE consists of 257 amino acids and has a predicted molecular mass of 29.4 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gly
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for 14-3-3 epsilon / YWHAE recombinant protein

Background: YWHAE, also known as 14-3-3 epsilon, mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 epsilon / YWHAE is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 epsilon / YWHAE is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. YWHAE interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 epsilon / YWHAE is implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 epsilon / YWHAE Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. This Binding generally results in the modulation of the activity of the binding partner.

Description: A DNA sequence encoding the human YWHAE (NP_006752.1) (Met 1-Gln 255) was expressed, with two additional amino acids (Gly & Pro) at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 5803225
• NCBI GeneID: 7531
• NCBI Accession #: NP_006752.1
• NCBI GenBank Nucleotide #: NM_006761.4
• UniProt Accession #: P62258; P29360; P42655; Q4VJB6; Q53XZ5; Q63631; Q7M4R4; B3KY71; D3DTH5
• Molecular Weight: 26,504 Da
• NCBI Official Full Name: 14-3-3 protein epsilon
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon
• NCBI Official Symbol: YWHAE
• NCBI Official Synonym Symbols: MDS; HEL2; MDCR; KCIP-1; 14-3-3E
• NCBI Protein Information: 14-3-3 protein epsilon
• UniProt Protein Name: 14-3-3 protein epsilon
• UniProt Gene Name: YWHAE
• UniProt Synonym Gene Names: 14-3-3E

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

14-3-3 epsilon: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: cell-cell adherens junction; cytoplasmic vesicle membrane; cytosol; focal adhesion; membrane

Molecular Function: enzyme binding; histone deacetylase binding; phosphoprotein binding; phosphoserine binding; potassium channel regulator activity; protein binding; protein heterodimerization activity; ubiquitin protein ligase binding

Biological Process: G2/M transition of mitotic cell cycle; negative regulation of caspase activity; substantia nigra development

Disease: Miller-dieker Lissencephaly Syndrome