Interferon-gamma Active Protein | IFN-gamma active protein

Human Interferon-gamma (IFN-gamma), E Coli

Cat. Num. AAA434174

• Gene Names: IFNG; IFG; IFI
• Purity: > 97% (by SDS-PAGE and HPLC)
• Synonyms: Interferon-gamma; Human Interferon-gamma (IFN-gamma); E Coli; Interferon-gamma (INF-gamma); human interferon-gamma; IFN-gamma active protein

• Host: Yeast
• Purity/Purification: > 97% (by SDS-PAGE and HPLC)
• Form/Format: Lyophilized from a 0.2mum filtered solution in 20mM Phosphate Buffer; Reconstitute with double distilled water at a concentration of no less than 10 mug/ml with 0.1% human serum albumin (A highly purified plant-derived human serum albumin is strongly su
• Sequence Length: 166

• Endotoxin Level: <=1 EU/mg, determined by the LAL method
• Biological Activity: Bioactivity is detected using WISH cell (a heteroploid human amnion cell line). The specific activity shall be not less than 2.0×107 IU/mg of protein.
• Preparation and Storage: Stable for 6-months from the date of shipment when kept at -20 degree C or -70 degree C. Upon reconstitution, it can be stored at 4 degree C for at least one month or -20 degree C for at least three months. Avoid repeated freeze-thaw cycles.

Related Product Information for IFN-gamma active protein

Description: Recombinant human IFN-gamma produced in E Coli. is a non-covalent Dimer non-glycosylated, containing 140 amino acids, and having a molecular mass of approximately 16.5kD.
Introduction: Interferon-gamma (IFN-gamma, also known as Type II interferon or immune interferon) is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-beta or the various IFN-alpha family proteins. Mature IFN-gamma exists as noncovalently-linked homodimers. Human IFN-gamma is highly species specific and is biologically active only in human and primate cells. IFN-gamma was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-gamma induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-gamma also augments TH1 cell expansion and may be required for TH1 cell differentiation. IFN-gamma exerts its biological activities by binding to specific cell surface receptors with high-affinity binding sites. The IFN-gamma receptor is present on almost all cell types except mature erythrocytes and has been cloned and characterized. The IFN-gamma receptor is structurally related to the recently cloned IL-10 receptor.

Product Categories/Family for IFN-gamma active protein

Cytokine Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 56786138
• NCBI GeneID: 3458
• NCBI Accession #: NP_000610.2
• NCBI GenBank Nucleotide #: NM_000619.2
• Molecular Weight: 19,348 Da
• NCBI Official Full Name: interferon gamma
• NCBI Official Synonym Full Names: interferon, gamma
• NCBI Official Symbol: IFNG
• NCBI Official Synonym Symbols: IFG; IFI
• NCBI Protein Information: interferon gamma; IFN-gamma; immune interferon
• UniProt Protein Name: Interferon gamma
• UniProt Gene Name: IFNG
• UniProt Entry Name: IFNG_HUMAN

NCBI Description

This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]

Uniprot Description

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.

Protein type: Membrane protein, integral; Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: interferon-gamma receptor binding; cytokine activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; positive regulation of osteoclast differentiation; apoptosis; positive regulation of interleukin-23 production; positive regulation of interleukin-12 production; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-1 beta secretion; positive regulation of killing of cells of another organism; sensory perception of mechanical stimulus; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; neutrophil chemotaxis; positive regulation of synaptic transmission, cholinergic; adaptive immune response; negative regulation of myelination; CD8-positive, alpha-beta T cell differentiation during immune response; unfolded protein response; response to virus; cytokine and chemokine mediated signaling pathway; positive regulation of tumor necrosis factor production; defense response to protozoan; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of interleukin-12 biosynthetic process; positive regulation of chemokine biosynthetic process; negative regulation of interleukin-17 production; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; cell motility; positive regulation of neuron differentiation; regulation of insulin secretion

Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Mycobacterium Tuberculosis, Susceptibility To; Tuberous Sclerosis 2; Human Immunodeficiency Virus Type 1, Susceptibility To